Bone Marrow Mesenchymal Stem Cell-Derived Microvesicles Protect Rat Pheochromocytoma PC12 Cells from Glutamate-Induced Injury via a PI3K/Akt Dependent Pathway
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- 作者：Shan-Shan Lin (1)
Bo Zhu (2)
Zi-Kuan Guo (3)
Guo-Zhi Huang (1)
Zi Wang (3)
Jin Chen (3)
Xiao-Juan Wei (3)
Qi Li (2) - 关键词：Mesenchymal stem cells ; Microvesicles ; Glutamate ; PI3K/Akt
- 刊名：Neurochemical Research
- 出版年：2014
- 出版时间：May 2014
- 年：2014
- 卷：39
- 期：5
- 页码：922-931
- 全文大小：
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Bo Zhu (2)
Zi-Kuan Guo (3)
Guo-Zhi Huang (1)
Zi Wang (3)
Jin Chen (3)
Xiao-Juan Wei (3)
Qi Li (2)
1. Department of Rehabilitation Medicine, Zhujiang Hospital, Southern Medical University, 253 Gongye Road, Guangzhou, 510282, China
2. Department of Orthopedics, Zhujiang Hospital, Southern Medical University, Guangzhou, China
3. Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing, China - ISSN：1573-6903
文摘
Studies have suggested that mesenchymal stem cells (MSCs) can protect neuronal cells from excitotoxicity, but the underlying mechanisms are still remaining elusive. In the study, we show that microvesicles released by rat bone marrow-derived MSCs (rBMSC-MVs) protect rat pheochromocytoma PC12 cells from glutamate-induced excitotoxicity. BMSC-MVs upregulate Akt phosphorylation and Bcl-2 expression, downregulate Bax expression, and reduce the cleavage of caspase-3 in glutamate-treated PC12 cells. Such protective effects are partially abrogated by inhibiting PI3K, indicating that rBMSC-MVs act via the PI3K/Akt pathway. Transplantation of rBMSC-MVs may, therefore, be a promising strategy to treat cerebral injury or some other neuronal diseases involving excitotoxicity.