Effects of two fast-setting calcium-silicate cements on cell viability and angiogenic factor release in human pulp-derived cells

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• 作者：Chooryung J. Chung ; Euiseong Kim ; Minju Song ; Jeong-Won Park ; Su-Jung Shin
• 关键词：Calcium ; silicate cement ; Fast setting ; Mineral trioxide aggregate ; Pozzolan cement ; Pulp cell
• 刊名：Odontology
• 出版年：2016
• 出版时间：May 2016
• 年：2016
• 卷：104
• 期：2
• 页码：143-151
• 全文大小：1,372 KB
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• 作者单位：Chooryung J. Chung (1)
  Euiseong Kim (2)
  Minju Song (3)
  Jeong-Won Park (3)
  Su-Jung Shin (3)
  
  1. Department of Orthodontics, College of Dentistry, Gangnam Severance Hospital, Yonsei University, Seoul, Korea
  2. Department of Conservative Dentistry, College of Dentistry, Yonsei University, Seoul, Korea
  3. Department of Conservative Dentistry, College of Dentistry, Gangnam Severance Hospital, Yonsei University, 211 Eonjuro, Gangnam-gu, Seoul, Korea
  
• 刊物类别：Medicine
• 刊物主题：Dentistry
  
• 出版者：Springer Japan
• ISSN：1618-1255

文摘

Mineral trioxide aggregate (MTA) is considered a pulp-capping agent of choice, but has the drawback of a long setting time. This study aimed to assess two different types of calcium-silicate cements as pulp-capping agents, by investigating their in vitro cytotoxicity and angiogenic effects in human pulp cells. ProRoot MTA, Endocem Zr, and Retro MTA were prepared as set or freshly mixed pellets. Human pulp-derived cells were grown in direct contact with these three cements, Dycal, or no cement, for 7 days. Initial cell attachment, viability, calcium release, and the levels of vascular endothelial growth factor (VEGF), angiogenin, and basic fibroblast growth factor (FGF-2) were evaluated statistically using a linear mixed model (P < 0.05). The biocompatibility of Retro MTA was similar to those of the control and ProRoot MTA. Endocem Zr groups showed fewer and more rounded cells after a 3-day culture; however, the initial cytotoxicity appeared transient. All test materials showed significant increases in calcium concentration compared with the control group (P < 0.05). VEGF and angiogenin levels in ProRoot MTA and Retro MTA groups were significantly higher than those in the Endocem Zr group (P < 0.05). FGF-2 levels were not significantly different between groups (P > 0.05). We demonstrate that Retro MTA, which has a short setting time, has similar biocompatibility and angiogenic effects on human pulp cells, and can therefore potentially be as effective in pulp capping as ProRoot MTA. Endocem Zr showed intermittent cytotoxicity and elicited lower levels of VEGF and angiogenin expression.