Biological characteristics of a specific brain metastatic cell line derived from human lung adenocarcinoma

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• 作者：Ling Hu (1)
  Junqing Zhang (1)
  Hongbin Zhu (1)
  Jie Min (1)
  Yingming Feng (1)
  Helong Zhang (1)
  
• 关键词：Lung cancer ; Metastases ; Vascular endothelial growth factors ; Matrix metalloproteinase ; 9 ; S100B ; Epidermal growth factor receptor
• 刊名：Medical Oncology
• 出版年：2010
• 出版时间：September 2010
• 年：2010
• 卷：27
• 期：3
• 页码：708-714
• 全文大小：510KB
• 参考文献：1. Ishikawa H, Satoh H, Kurishima K, et al. Lung cancer with synchronous brain and bone metastasis. Clin Oncol (R Coll Radiol). 2000;12:136鈥?.
  2. Paget S. The distribution of secondary growths in cancer of the breast. 1889. Cancer Metastasis Rev. 1989;8:98鈥?01.
  3. Kim LS, Huang S, Lu W, et al. Vascular endothelial growth factor expression promotes the growth of breast cancer brain metastases in nude mice. Clin Exp Metastasis. 2004;21:107鈥?8. CrossRef
  4. Sierra A, Price JE, Garcia-Ramirez M, et al. Astrocyte-derived cytokines contribute to the metastatic brain specificity of breast cancer cells. Lab Invest. 1997;77:357鈥?8.
  5. Lee TH, Avraham HK, Jiang S, et al. Vascular endothelial growth factor modulates the transendothelial migration of MDA-MB-231 breast cancer cells through regulation of brain microvascular endothelial cell permeability. J Biol Chem. 2003;278:5277鈥?4. CrossRef
  6. Yano S, Shinohara H, Herbst RS, et al. Expression of vascular endothelial growth factor is necessary but not sufficient for production and growth of brain metastasis. Cancer Res. 2000;60:4959鈥?7.
  7. Chantrain CF, Shimada H, Jodele S, et al. Stromal matrix metalloproteinase-9 regulates the vascular architecture in neuroblastoma by promoting pericyte recruitment. Cancer Res. 2004;64:1675鈥?6. CrossRef
  8. Coussens LM, Tinkle CL, Hanahan D, et al. MMP-9 supplied by bone marrow-derived cells contributes to skin carcinogenesis. Cell. 2000;103:481鈥?0. CrossRef
  9. Pirker R, Pereira JR, Szczesna A, et al. Cetuximab plus chemotherapy in patients with advanced non-small-cell lung cancer (FLEX): an open-label randomised phase III trial. Lancet. 2009;373:1525鈥?1. CrossRef
  10. Mendes O, Kim HT, Stoica G. Expression of MMP2, MMP9 and MMP3 in breast cancer brain metastasis in a rat model. Clin Exp Metastasis. 2005;22:237鈥?6. CrossRef
  11. Zhang S, Yang X, Zhao A. Clinical analysis of combination therapy for 252 patients of lung cancer with brain metastasis. Zhonghua Jie He He Hu Xi Za Zhi. 1999;22:475鈥?.
  12. RR R, DM B, DJ W. Structure of the negative regulatory domain of p53 bound to S100B(betabeta). Nat Struct Biol. 2000;7:525鈥?. CrossRef
  13. Lin J, Yang Q, Yan Z, et al. Inhibiting S100B restores p53 levels in primary malignant melanoma cancer cells. J Biol Chem. 2004;279:34071鈥?. CrossRef
  14. Arcuri C, Bianchi R, Brozzi F, et al. S100B increases proliferation in PC12 neuronal cells and reduces their responsiveness to nerve growth factor via Akt activation. J Biol Chem. 2005;280:4402鈥?4. CrossRef
  15. Millward TA, Heizmann CW, Schafer BW, et al. Calcium regulation of Ndr protein kinase mediated by S100 calcium-binding proteins. EMBO J. 1998;17:5913鈥?2. CrossRef
  16. Nicholson RI, Gee JM, Harper ME. EGFR and cancer prognosis. Eur J Cancer. 2001;37(Suppl 4):S9鈥?5. CrossRef
  17. Olayioye MA, Neve RM, Lane HA, et al. The ErbB signaling network: receptor heterodimerization in development and cancer. EMBO J. 2000;19:3159鈥?7. CrossRef
  18. Yarden Y, Sliwkowski MX. Untangling the ErbB signalling network. Nat Rev Mol Cell Biol. 2001;2:127鈥?7. CrossRef
  19. Zhang J-q, Zhang H-l, Feng Y-m. Establishment of highly brain metastatic lung cancer cell sub-clone and brain metastatic animal model. J Mod Oncol. 2007;15:48鈥?.
  20. Hirashima Y, Kobayashi H, Suzuki M, et al. Transforming growth factor-beta1 produced by ovarian cancer cell line HRA stimulates attachment and invasion through an up-regulation of plasminogen activator inhibitor type-1 in human peritoneal mesothelial cells. J Biol Chem. 2003;278:26793鈥?02. CrossRef
  21. Abratt RP, de Groot M, Willcox PA. Resection of a solitary brain metastasis in a patient with small cell lung cancer鈥攍ong-term survival. Eur J Cancer. 1995;31A:419. CrossRef
  22. Chambers AF, Groom AC, MacDonald IC. Dissemination and growth of cancer cells in metastatic sites. Nat Rev Cancer. 2002;2:563鈥?2. CrossRef
  23. Schuette W. Treatment of brain metastases from lung cancer: chemotherapy. Lung Cancer. 2004;45(Suppl 2):S253鈥?. CrossRef
  24. Fidler IJ. The organ microenvironment and cancer metastasis. Differentiation. 2002;70:498鈥?05. CrossRef
  25. He BP, Wang JJ, Zhang X, et al. Differential reactions of microglia to brain metastasis of lung cancer. Mol Med. 2006;12:161鈥?0. CrossRef
  26. Vogelbaum MA, Mazzone P, Masaryk T. Low serum S100B levels in patients with newly diagnosed lung cancer correlate with an absence of brain metastases on MIR. / Neuro-Oncology 2005;330鈥?31.
  27. Markowitz J, Mackerell AD Jr, Carrier F. Design of inhibitors for S100B. Curr Top Med Chem. 2005;5:1093鈥?08. CrossRef
  28. Shi AH, Zhu GY, Yu R. Whole brain irradiation for non-small-cell lung cancer with brain metastasis. Zhonghua Zhong Liu Za Zhi. 2007;29:545鈥?.
  29. Talmadge J, Talmadge JE, Wolman SR, Fidler IJ. Evidence for the clonal origin of spontaneous metastases. Science. 1982;217:361鈥?. CrossRef
  30. Marchetti D, Denkins Y, Reiland J, et al. Brain-metastatic melanoma: a neurotrophic perspective. Pathol Oncol Res. 2003;9:147鈥?8. CrossRef
  31. Matsumoto S, Takahashi K, Iwakawa R, et al. Frequent EGFR mutations in brain metastases of lung adenocarcinoma. Int J Cancer. 2006;119:1491鈥?. CrossRef
  
• 作者单位：Ling Hu (1)
  Junqing Zhang (1)
  Hongbin Zhu (1)
  Jie Min (1)
  Yingming Feng (1)
  Helong Zhang (1)
  
  1. Department of Oncology, Tangdu Hospital, The Fourth Military Medical University, Xi鈥檃n, 710038, China
  

文摘

To study the expression of VEGF, MMP-9, EGFR, and S100B in a highly brain metastases sub-clone cell line, PC14/B. The in vitro metastases-related behaviors of PC14 /B cells, such as adhesion to extracellular matrix (ECM), migration, and invasion were determined and compared with primary PC14 cells and A549 cells that do not metastasize to brain. The expression of vascular epithelial growth factor (VEGF), matrix metalloproteinase 9 (MMP-9), S100B, and epidermal growth factor receptor (EGFR) in the above three cell lines were measured by immunohistochemical staining and Western blot assay.The PC14/B cells have enhanced abilities of adhesion, migration, and invasion than PC14 cells and A549 cells. The expression levels of VEGF and MMP-9 in PC14/B cells are much higher than in PC14 and A549 cells. Two protein polymers of S100B are expressed specially in PC14/B cells. The expression of EGFR has a significant lower level in PC14 cells than in the other two cell lines. The increased expression of VEGF and MMP-9 may lead to the enhancement of adhesion, migration, and invasion of PC14/B cells. The expression of EGFR in PC14/B cells may have negative correlation with their capacities of metastasizing to brain. The specific expression of S100B in PC14/B cells strongly suggest that S100B might be a potential target for developing new therapy to brain metastases of lung cancer.