Chemical composition of Enterococcus faecalis in biofilm cells initiated from different physiologic states
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- 作者：Hongyan Liu (1) (2)
Qiong Xu (1) (2)
Lijun Huo (1) (2)
Xi Wei (1) (2)
Junqi Ling (1) (2) - 刊名：Folia Microbiologica
- 出版年：2014
- 出版时间：September 2014
- 年：2014
- 卷：59
- 期：5
- 页码：447-453
- 全文大小：3,137 KB
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Qiong Xu (1) (2)
Lijun Huo (1) (2)
Xi Wei (1) (2)
Junqi Ling (1) (2)
1. Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Sun Yat-sen University, No. 56 Lingyuanxi Road, Guangzhou, 510055, Guangdong, China
2. Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, No. 56 Lingyuanxi Road, Guangzhou, 510055, Guangdong, People’s Republic of China - ISSN：1874-9356
文摘
Enterococcus faecalis is a ubiquitous bacterium of the gut that is observed in persistent periradicular infections. Its pathogenicity is associated with biofilm formation and the ability to survive under nutrient-poor (starvation) conditions. However, characteristics of chemical composition of biofilm cells developed by starved E. faecalis cells remain poorly understood. In this study, E. faecalis cells in exponential, stationary, and starvation phases were prepared and separately cultured to form biofilms. Confocal laser scanning microscopy was performed to verify biofilm formation. Raman microscopy was used to investigate the chemical composition of cells within the biofilms. Compared to cells in exponential or stationary phase, starved cells developed biofilms with fewer culturable cells (P--.05). Raman analysis revealed that cells produced in the biofilms from starved planktonic cells contained more protein and less nucleic acids than either the corresponding planktonic cells or the cells in biofilms from planktonic cells in exponential or stationary phases, suggesting that biofilm-grown cells from the starvation phase were characterized by increased synthesis of proteins and decreased nucleic acids. This study provides an insight into the chemical composition of biofilm cells developed by starved E. faecalis.