Isolation and characterization of a wheat F8-1 homolog required for physiological male sterility induced by a chemical hybridizing agent (CHA) SQ-1

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• 作者：Yulong Song ; Junwei Wang ; Gaisheng Zhang ; Xinliang Zhao ; Pengfei Zhang ; Na Niu…
• 关键词：Triticum aestivum L. ; Physiological male sterility ; Barley stripe mosaic virus ; Pollen allergen ; Subcellular localization
• 刊名：Euphytica
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：205
• 期：3
• 页码：707-720
• 全文大小：3,750 KB
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• 作者单位：Yulong Song (1) (2) (3) (4) (5)
  Junwei Wang (1) (2) (3) (4) (5)
  Gaisheng Zhang (1) (2) (3) (4) (5)
  Xinliang Zhao (1) (2) (3) (4) (5)
  Pengfei Zhang (1) (2) (3) (4) (5)
  Na Niu (1) (2) (3) (4) (5)
  Shoucai Ma (1) (2) (3) (4) (5)
  
  1. College of Agronomy, Northwest A&F University, Yangling, Shaanxi, People’s Republic of China
  2. National Yangling Agricultural Biotechnology and Breeding Center, Yangling, Shaanxi, People’s Republic of China
  3. Yangling Branch of State Wheat Improvement Center, Yangling, Shaanxi, People’s Republic of China
  4. Wheat Breeding Engineering Research Center, Ministry of Education, Yangling, Shaanxi, People’s Republic of China
  5. Key Laboratory of Crop Heterosis of Shaanxi Province, Yangling, 712100, Shaanxi, People’s Republic of China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Physiology
  Plant Sciences
  Ecology
  
• 出版者：Springer Netherlands
• ISSN：1573-5060

文摘

We investigated the relationship between a pollen-specific protein homolog and pollen abortion in an SQ-1-induced male sterile line of wheat (Triticum aestivum L.). We previously determined that EST S414 (Ta20678) was obviously down-regulated in a line of Xinong 1376 wheat in which male sterility was induced by SQ-1 (a chemical hybridizing agent). In the present study, we obtained the full sequence of F8-1 from wheat. The open reading frame sequence of the F8-1 gene was 495 bp, encoding 165 amino acid residues with a predicted molecular weight of 18.2759 kDa and an isoelectric point of 4.86. This sequence was identified as a Secale cereale × Triticum durum F8-4 homolog with 100 % identity. The amino acids represent a conserved domain in pollen Ole e I that contains the consensus sequence Q-G-R-V-Y-C-D-T-C-R. The protein has a close evolutionary relationship with orthologs in S. cereale × T. durum and Triticum urartu, consisting of 9.09 % alpha helix, 24.85 % extended strand, and 66.06 % random coil. F8-1 was only expressed in anthers during the binucleate and trinucleate stages and expression gradually decreased from the binucleate stage to the trinucleate stage. The expression of F8-1 gene of physiological male sterility 1376 (PHYMS-1376) was lower than that of 1376 at both the binucleate and trinucleate stages. Subcellular localization showed that the F8-1 protein was expressed at the nucleus. RNAi tests showed that down-regulation of the F8-1 was associated with an increase in the percentage of pollen abortion. Therefore, F8-1 is a positive regulator of physiological male sterility. Keywords Triticum aestivum L. Physiological male sterility Barley stripe mosaic virus Pollen allergen Subcellular localization