Sustained mitogenic effect on K562 human chronic myelogenous leukemia cells by dietary lectin, jacalin

详细信息    查看全文

• 作者：V. Lavanya ; Neesar Ahmed ; Md Khurshid Alam Khan ; Shazia Jamal
• 关键词：Lectins ; Jacalin ; Cell proliferation ; K562 erythroleukemia cells ; Thomsen ; Friendenreich disaccharide ; Pro inflammatory cytokines
• 刊名：Glycoconjugate Journal
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：33
• 期：6
• 页码：877-886
• 全文大小：
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biochemistry, general; Pathology;
• 出版者：Springer US
• ISSN：1573-4986
• 卷排序：33

文摘

Dietary lectins have been shown to affect the proliferation of human cancer cell lines. The anti-proliferative effects of lectins from varied sources have been extensively studied and in some cases, the underlying mechanism has been explored. Except for peanut agglutinin (PNA), the mitogenic effects of no other lectins have been studied in detail. In the present study, we have shown that jacalin, lectin purified from jackfruit (Artocarpus integrifolia) seeds act as a mitogen for K562, the Bcr-Abl expressing erythroleukemia cell line (K562) and the effect was found to be dose dependent. K562 cells remained in the proliferative state for a longer period even after the withdrawal of jacalin stimulation, thus jacalin was found to induce sustained mitogenic effect on K562 cells. Further, conditioned media from K562 cells treated with jacalin were observed to have the similar mitogenic effect even in the presence of galactose. Importantly, galactose which is a known ligand for jacalin will interact with functionally active jacalin present in the conditioned media and neutralise its effect. In addition, jacalin treatment also resulted in increased mRNA expression levels of pro-inflammatory cytokines including IL-1β, IL-6 and IFN-γ. Our results indicate that jacalin induces secretion of soluble molecules, which maybe responsible for this observed increased proliferation of K562 cells.