The function and mechanism of COX-2 in angiogenesis of gastric cancer cells

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• 作者：Liping Yao (1)
  Fei Liu (1)
  Liu Hong (1)
  Li Sun (1)
  Shuhui Liang (1)
  Kaichun Wu (1)
  Daiming Fan (1)
  
• 刊名：Journal of Experimental & Clinical Cancer Research
• 出版年：2011
• 出版时间：December 2011
• 年：2011
• 卷：30
• 期：1
• 全文大小：602KB
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• 作者单位：Liping Yao (1)
  Fei Liu (1)
  Liu Hong (1)
  Li Sun (1)
  Shuhui Liang (1)
  Kaichun Wu (1)
  Daiming Fan (1)
  
  1. State Key Laboratory of Cancer Biology and Xijing Hospital of Digestive Diseases, Fourth Military Medical University, 15 West Changle Road, Xi鈥檃n, 710032, PR China
  
• ISSN：1756-9966

文摘

Background Here we aimed to investigate the effect of COX-2 siRNA on proliferation and angiogenesis of gastric cancer cells. Methods The gastric cancer cell line SGC7901 was transfected with COX-2 siRNA, then the growth and angiogenesis of cells were detected by in vitro and in vivo assay. Human microarray, RT-PCR and western blot were used to identify differentially expressed angiogenesis-related molecules in cells with decreased expression of COX-2. Results Down-regulation of COX-2 could significantly inhibit the in vitro and in vivo growth of gastric cancer cells, and suppress the migration and tube formation of human umbilical vein endothelial cells. Totally 23 angiogenesis-related molecules were found involved in COX-2-induced angiogenesis suppression. The results of RT-PCR and western blot showed that down-regulation of COX-2 might inhibit VEGF, Flt-1, Flk-1/KDR, angiopoietin-1, tie-2, MMP2 and OPN. Conclusions COX-2 might mediate tumor angiogenesis and growth, and could be considered as a target for gastric cancer therapy.