USP33, a new player in lung cancer, mediates Slit-Robo signaling

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• 作者：Pushuai Wen (1) (5) (6)
  Ruirui Kong (1)
  Jianghong Liu (1)
  Li Zhu (1)
  Xiaoping Chen (2)
  Xiaofei Li (3)
  Yongzhan Nie (4)
  Kaichun Wu (4)
  Jane Y. Wu (1) (2)
  
• 关键词：USP33 ; Slit ; Robo ; lung cancer ; Slit ; Robo signaling ; tumor suppressor ; prognostic marker
• 刊名：Protein & Cell
• 出版年：2014
• 出版时间：September 2014
• 年：2014
• 卷：5
• 期：9
• 页码：704-713
• 全文大小：835 KB
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• 作者单位：Pushuai Wen (1) (5) (6)
  Ruirui Kong (1)
  Jianghong Liu (1)
  Li Zhu (1)
  Xiaoping Chen (2)
  Xiaofei Li (3)
  Yongzhan Nie (4)
  Kaichun Wu (4)
  Jane Y. Wu (1) (2)
  
  1. State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China
  5. University of Chinese Academy of Sciences, Beijing, 100049, China
  6. Department of Pathophysiology, Liaoning Medical University, Jinzhou, 121001, China
  2. Department of Neurology, Center for Genetic Medicine, Lurie Cancer Center, Northwestern University Feinberg School of Medicine, 303 E. Chicago Ave., Chicago, IL, 60611, USA
  3. Department of Thoracic Surgery, Tangdu Hospital, Fourth Military Medical University, Xi鈥檃n, 710038, China
  4. State Key Laboratory of Cancer Biology and Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi鈥檃n, 710032, China
  
• ISSN：1674-8018

文摘

Ubiquitin specific protease 33 (USP33) is a multifunctional protein regulating diverse cellular processes. The expression and role of USP33 in lung cancer remain unexplored. In this study, we show that USP33 is down-regulated in multiple cohorts of lung cancer patients and that low expression of USP33 is associated with poor prognosis. USP33 mediates Slit-Robo signaling in lung cancer cell migration. Downregulation of USP33 reduces the protein stability of Robo1 in lung cancer cells, providing a previously unknown mechanism for USP33 function in mediating Slit activity in lung cancer cells. Taken together, USP33 is a new player in lung cancer that regulates Slit-Robo signaling. Our data suggest that USP33 may be a candidate tumor suppressor for lung cancer with potential as a prognostic marker.