Simultaneous Determination of Five Major Compounds in Polygonum cuspidatum by HPLC
详细信息 查看全文
- 作者：Wenting Zhang ; Ying Jia ; Qinwei Huang ; Qing Li and Kaishun Bi
- 关键词：Column liquid chromatography ; Resveratrol ; polydatin ; anthraglycoside B ; emodin ; physcion ; Polygonum cuspidatum
- 刊名：Chromatographia
- 出版年：2007
- 出版时间：November, 2007
- 年：2007
- 卷：66
- 期：9-10
- 页码：685-689
- 全文大小：355 KB
- 刊物类别：Chemistry and Materials Science
- 刊物主题：Chemistry
Analytical Chemistry
Organic Chemistry
Pharmacy
Biochemistry
Plant Sciences
Measurement Science and Instrumentation - 出版者：Vieweg Verlag
- ISSN：1612-1112
文摘
An RP-HPLC method was developed for the first time to simultaneously determine five major compounds in Polygonum cuspidatum, namely resveratrol, polydatin, anthraglycoside B, emodin and physcion with UV detection at 306 nm. The column was an Agilent Zorbax SB-C18 (250 × 4.6 mm i.d., 5 μm). The separation was carried out with a gradient program. The mobile phase was acetonitrile–water (containing 0.1% formic acid) at a flow rate of 1.0 mL min?1. The standard curve was rectilinear in the range of 2.04–62.96 μg mL?1 (r = 0.9998) for resveratrol, 20.13–239.7 μg mL?1 (r = 0.9998) for polydatin, 7.19–71.92 μg mL?1 (r = 1.0000) for anthraglycoside B, 2.68–83.68 μg mL?1 (r = 0.9998) for emodin and 0.60–14.37 μg mL?1 (r = 0.9997) for physcion. The recoveries of the markers were 96.0, 106.5, 97.8, 97.9 and 98.1%, respectively. The relative standard deviation of intra-day and inter-day were less than 5.0 and 2.3%. This method was simple, accurate and reproducible. The developed method was successfully applied to analyze five compounds in P. cuspidatum of 20 commercial brands.