Identification of silicateins in freshwater sponge Lubomirskia baicalensis
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- 作者：O. V. Kaluzhnaya ; A. S. Belikova ; E. P. Podolskaya ; A. G. Krasko ; W. E. G. M¨¹ller and S. I. Belikov
- 关键词：sponge ; Lubomirskia baicalensis ; biogenic silica ; silicatein ; intron ; exon structure ; mass spectrometry ; tryptic digest
- 刊名：Molecular Biology
- 出版年：2007
- 出版时间：August, 2007
- 年：2007
- 卷：41
- 期：4
- 页码：554-561
- 全文大小：456 KB
文摘
Siliceous spicules of Baikal freshwater sponge Lubomirskia baicalensis contain several proteins, including silicateins. Analysis of a L. baicalensis cDNA library revealed four different mRNAs coding for proteins related to marine sponge silicatein α (α1, α2, α3, and α4). The intron-exon structure was determined forthe genomic α1 silicatein gene. The gene is 1988 bp from the initiation to the termination codon and consists of six intron (total size 1007 bp) and seven exons (total size 981 bp). Mass spectrometry of a tryptic digest of spicule proteins revealed peptides of two silicateins α.