Expression pattern of peptide and amino acid genes in digestive tract of transporter juvenile turbot (Scophthalmus maximus L.)

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• 作者：Dandan Xu ; Gen He ; Kangsen Mai ; Huihui Zhou ; Wei Xu…
• 关键词：turbot ; digestive tract ; amino acid ; peptide ; transporter ; expression pattern
• 刊名：Journal of Ocean University of China
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：15
• 期：2
• 页码：334-340
• 全文大小：445 KB
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• 作者单位：Dandan Xu (1)
  Gen He (1)
  Kangsen Mai (1)
  Huihui Zhou (1)
  Wei Xu (1)
  Fei Song (1)
  
  1. Key Laboratory of Aquanutrition of Ministry of Agriculture, Ocean University of China, Qingdao, 266003, P. R. China
  
• 刊物主题：Oceanography; Meteorology;
• 出版者：Springer Berlin Heidelberg
• ISSN：1993-5021

文摘

Turbot (Scophthalmus maximus L.), a carnivorous fish species with high dietary protein requirement, was chosen to examine the expression pattern of peptide and amino acid transporter genes along its digestive tract which was divided into six segments including stomach, pyloric caeca, rectum, and three equal parts of the remainder of the intestine. The results showed that the expression of two peptide and eleven amino acid transporters genes exhibited distinct patterns. Peptide transporter 1 (PepT1) was rich in proximal intestine while peptide transporter 2 (PepT2) was abundant in distal intestine. A number of neutral and cationic amino acid transporters expressed richly in whole intestine including B0-type amino acid transporter 1 (B0AT1), L-type amino acid transporter 2 (LAT2), T-type amino acid transporter 1 (TAT1), proton-coupled amino acid transporter 1 (PAT1), y+L-type amino acid transporter 1 (y+LAT1), and cationic amino acid transporter 2 (CAT2) while ASC amino acid transporter 2 (ASCT2), sodium-coupled neutral amino acid transporter 2 (SNAT2), and y+L-type amino acid transporter 2 (y+LAT2) abundantly expressed in stomach. In addition, system b0,+ transporters (rBAT and b0,+AT) existed richly in distal intestine. These findings comprehensively characterized the distribution of solute carrier family proteins, which revealed the relative importance of peptide and amino acid absorption through luminal membrane. Our findings are helpful to understand the mechanism of the utilization of dietary protein in fish with a short digestive tract. Keywords turbot digestive tract amino acid peptide transporter expression pattern