Application of TaqMan qPCR for the detection and monitoring of Naegleria species in reservoirs used as a source for drinking water
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- 作者：Po-Min Kao ; Bing-Mu Hsu ; Tsui-Kang Hsu ; Yi-Chou Chiu…
- 关键词：Naegleria ; Reservoir ; Culture ; PCR ; TaqMan real ; time qPCR
- 刊名：Parasitology Research
- 出版年：2014
- 出版时间：October 2014
- 年：2014
- 卷：113
- 期：10
- 页码：3765-3771
- 全文大小：295 KB
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Bing-Mu Hsu (1)
Tsui-Kang Hsu (2) (3)
Yi-Chou Chiu (4)
Chung-Liang Chang (5)
Wen-Tsai Ji (1)
Shih-Wei Huang (6)
Cheng-Wei Fan (1)
1. Department of Earth and Environmental Sciences, National Chung Cheng University, No.168, Sec. 1, University Rd., Minhsiung Township, Chiayi, 621, Taiwan, Republic of China
2. Department of Ophthalmology, Cheng Hsin General Hospital, No.45, Chenghsin St., Paitou Dist., Taipei, 112, Taiwan, Republic of China
3. Department of Life Science, Institute of Molecular Biology, National Chung Cheng University, No.168, Sec. 1, University Rd., Minhsiung Township, Chiayi, 621, Taiwan, Republic of China
4. General Surgery, Surgical Department, Cheng Hsin General Hospital, No.45, Chenghsin St., Paitou Dist., Taipei, 112, Taiwan, Republic of China
5. Department of Environmental Engineering and Health, Yuanpei University of Science and Technology, No.306, Yuanpei St., Hsinchu, 300, Taiwan, Republic of China
6. Center for General Education, Cheng Shiu University, No.840, Chengcing Rd., Niaosong Dist., Kaohsiung, 833, Taiwan, Republic of China - ISSN：1432-1955
文摘
Naegleria spp. can be found in the natural aquatic environments. Naegleria fowleri can cause fatal infections in the central nervous system in humans and animals, and the most important source of infection is through direct water contact. In this study, PCR of 5.8S ribosomal RNA (rRNA) gene and internal transcribed spacer (ITS) region was performed in order to identify Naegleria isolates and quantify the Naegleria spp. by TaqMan real-time quantitative PCR in reservoir water samples. The occurrence of Naegleria spp. was investigated in 57 water samples from reservoirs with culture and PCR positive in 2 of them (3.5?%), respectively. The total detection rate was 7.0?% (4/ 57) for Naegleria spp. The identified species included Naegleria spp., Naegleria canariensis, and Naegleria clarki. N. fowleri was not found in Taiwan’s reservoirs used for drinking purposes. The concentrations of Naegleria spp. in detected positive reservoir water samples were in the range of 599 and 3.1?×-03?cells/L. The presence or absence of Naegleria spp. within the reservoir water samples showed significant difference with the levels of water temperature. The presence of Naegleria spp. in reservoirs considered a potential public health threat if pathogenic species exist in reservoirs.