Human β-defensin-2 production from lass="a-plus-plus">S. cerevisiae using the repressible lass="a-plus-plus">MET17 promoter
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- 作者：Thea S. B. Møller ; Joanna Hay ; Malcolm J. Saxton ; Karen Bunting…
- 关键词：MET17 promoter ; Saccharomyces cerevisiae ; Yeast ; Human β ; defensin ; 2
- 刊名：Microbial Cell Factories
- 出版年：2017
- 出版时间：December 2017
- 年：2017
- 卷：16
- 期：1
- 全文大小：1858KB
- 刊物类别：Chemistry and Materials Science
- 刊物主题：Applied Microbiology; Biotechnology; Microbiology; Microbial Genetics and Genomics; Enzymology; Genetic Engineering;
- 出版者：BioMed Central
- ISSN：1475-2859
- 卷排序：16
文摘
BackgroundBaker’s yeast Saccharomyces cerevisiae is a proven host for the commercial production of recombinant biopharmaceutical proteins. For the manufacture of heterologous proteins with activities deleterious to the host it can be desirable to minimise production during the growth phase and induce production late in the exponential phase. Protein expression by regulated promoter systems offers the possibility of improving productivity in this way by separating the recombinant protein production phase from the yeast growth phase. Commonly used inducible promoters do not always offer convenient solutions for industrial scale biopharmaceutical production with engineered yeast systems.