Development of polymorphic microsatellite markers of the common toad, Duttaphrynus melanostictus useful for genetic studies

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• 作者：S. Jegath Janani (3)
  Richa Sharma (3)
  Karthikeyan Vasudevan (1)
  Sushil K. Dutta (2)
  Ramesh K. Aggarwal (3)
  
• 关键词：Duttaphrynus melanostictus ; Asian common toad ; Microsatellite markers ; Conservation/population genetics ; Cross ; species transferability
• 刊名：Conservation Genetics Resources
• 出版年：2013
• 出版时间：June 2013
• 年：2013
• 卷：5
• 期：2
• 页码：537-540
• 全文大小：210KB
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• 作者单位：S. Jegath Janani (3)
  Richa Sharma (3)
  Karthikeyan Vasudevan (1)
  Sushil K. Dutta (2)
  Ramesh K. Aggarwal (3)
  
  3. Centre for Cellular and Molecular Biology (CSIR-CCMB), Uppal Road, Tarnaka, Hyderabad, 500007, Andhra Pradesh, India
  1. Wildlife Institute of India, P.O. Box 18, Dehradun, 248 001, India
  2. P.G. Department of Zoology, North Orissa University, Baripada, 757003, India
  

文摘

Asian common toad, Duttaphrynus melanostictus, is a widespread habitat generalist species of Indian-subcontinent and South-East Asia. Here we report, 14 new microsatellite markers (SSRs) for this toad, developed from a SSR-enriched genomic library, and validated using 44 unrelated samples of Asian toad and ~20 samples belonging to four congeners. Nine SSRs were highly polymorphic; amplified 6-2 alleles/marker with H o and PIC ranging from 0.477 to 0.955 and 0.518 to 0.918, respectively, while one was found to be monomorphic. Of the remaining four SSRs, two (DMccmb02, DMccmb17) amplified only two apparently fixed alleles in all samples, while the other two (DMccmb22, DMccmb29) amplified 1-alleles/sample; these four SSRs probably represent duplicated loci. Interestingly, all the 14 SSRs showed cross-species transferability to varying extent, with four SSRs showing 100?% transferability. It is hoped that these SSRs will greatly facilitate population genetic studies on the Asian toad species across their geographic distribution.