Phenotypic characterisation of Saccharomyces spp. yeast for tolerance to stresses encountered during fermentation of lignocellulosic residues to produce bioethanol
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- 作者：Tithira T Wimalasena (1)
Darren Greetham (1)
Marcus E Marvin (2)
Gianni Liti (3)
Yogeshwar Chandelia (1)
Andrew Hart (1)
Edward J Louis (2)
Trevor G Phister (1) (5)
Gregory A Tucker (1)
Katherine A Smart (1) (4) - 关键词：Saccharomyces spp. ; Phenotypic microarray ; Bioethanol ; Fermentation
- 刊名：Microbial Cell Factories
- 出版年：2014
- 出版时间：December 2014
- 年：2014
- 卷：13
- 期：1
- 全文大小：596 KB
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Darren Greetham (1)
Marcus E Marvin (2)
Gianni Liti (3)
Yogeshwar Chandelia (1)
Andrew Hart (1)
Edward J Louis (2)
Trevor G Phister (1) (5)
Gregory A Tucker (1)
Katherine A Smart (1) (4)
1. Bioenergy & Brewing Science, School of Biosciences, Sutton Bonington Campus, University of Nottingham, Loughborough, Leicestershire, LE12 6RD, UK
2. Centre for Genetic Architecture of Complex Traits, Department of Genetics, University of Leicester, Adrian Building, Leicester, LE1 7RH, UK
3. Faculty of Medicine, Institute for Research on Cancer and Aging, 28, Avenue De Valombrose, 06107, Nice, Cedex-02, France
5. Pepsico Int, 4, Leycroft Road, Leicester, LE4 1ET, UK
4. SABMiller plc, SABMiller House, Woking, Church Street West, Surrey, GU21 6HS, UK - ISSN：1475-2859
文摘
Background During industrial fermentation of lignocellulose residues to produce bioethanol, microorganisms are exposed to a number of factors that influence productivity. These include inhibitory compounds produced by the pre-treatment processes required to release constituent carbohydrates from biomass feed-stocks and during fermentation, exposure of the organisms to stressful conditions. In addition, for lignocellulosic bioethanol production, conversion of both pentose and hexose sugars is a pre-requisite for fermentative organisms for efficient and complete conversion. All these factors are important to maximise industrial efficiency, productivity and profit margins in order to make second-generation bioethanol an economically viable alternative to fossil fuels for future transport needs. Results The aim of the current study was to assess Saccharomyces yeasts for their capacity to tolerate osmotic, temperature and ethanol stresses and inhibitors that might typically be released during steam explosion of wheat straw. Phenotypic microarray analysis was used to measure tolerance as a function of growth and metabolic activity. Saccharomyces strains analysed in this study displayed natural variation to each stress condition common in bioethanol fermentations. In addition, many strains displayed tolerance to more than one stress, such as inhibitor tolerance combined with fermentation stresses. Conclusions Our results suggest that this study could identify a potential candidate strain or strains for efficient second generation bioethanol production. Knowledge of the Saccharomyces spp. strains grown in these conditions will aid the development of breeding programmes in order to generate more efficient strains for industrial fermentations.