Role of Se(VI) in counteracting oxidative damage in m class="a-plus-plus">Brassica junceam> L. under Cr(VI) stress

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• 作者：Neha Handa ; Sukhmeen Kaur Kohli ; Ashwani Kumar Thukral…
• 关键词：Brassica juncea ; Heavy metal stress ; Oxidative damage ; Reactive oxygen species ; Selenium
• 刊名：Acta Physiologiae Plantarum
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：39
• 期：2
• 全文大小：
• 刊物主题：Plant Physiology; Plant Genetics & Genomics; Plant Biochemistry; Plant Pathology; Plant Anatomy/Development; Agriculture;
• 出版者：Springer Berlin Heidelberg
• ISSN：1861-1664
• 卷排序：39

文摘

Chromium (Cr) is considered to be one of the major environmental hazards and poses a threat to both plant and animal health. Selenium (Se), however, has been recognized as an essential micronutrient in plants. To understand the role of Se(VI) in oxidative stress management and regulation of antioxidative defence mechanism against heavy metal stress, the seedlings of Brassica juncea L. were raised in Petri plates containing nutrient media supplemented with only with Se(VI) and Cr(VI), or their combination. It was observed that of Cr(VI) causes an increase in reactive oxygen species (ROS) in the seedlings leading to oxidative stress. Histological studies using confocal and visible microscopy confirmed the biochemical results. Supplementation of up to 4 µM of Se(VI) to media containing 300 µM of Cr(VI) reduced the contents of ROS and increased enzymatic and non-enzymatic antioxidants in the seedlings. At a concentration of 6 µM, however, Se(VI) was toxic. The results suggested that at appropriate concentrations, the exogenous application of Se(VI) enabled the B. juncea seedlings to counteract the effects of Cr(VI), thereby increasing the resistance of plants.