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- 作者：Ranjeet Kaur ; Anirban Chakraborty…
- 关键词：Wild rice genotype ; Soil water stress ; Inducible promoter ; Late embryogenesis abundant protein ; Oryza nivara ; IR20
- 刊名：Journal of Plant Biochemistry and Biotechnology
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：26
- 期：1
- 页码：14-26
- 全文大小：
- 刊物主题：Life Sciences, general; Plant Biochemistry; Protein Science; Receptors; Cell Biology;
- 出版者：Springer India
- ISSN：0974-1275
- 卷排序：26
文摘
Identification and characterization of plant promoters from wild rice genotypes showing inducible expression under soil water stress (SWS) is desirable for transgene expression to generate stress tolerant rice cultivars. A comparative expression profiling of Wsi18, a group 3 LEA gene, revealed differential response under SWS conditions between modern cultivated rice (IR20) and its wild progenitor (Oryza nivara). Wsi18 promoter from O. nivara showed enhanced inducible expression of the reporter gusA gene, encoding β-glucuronidase, in transgenic rice plants in comparison to similar promoter from IR20. Deletion analysis unravelled the cis-acting regulatory elements minimally required for optimal expression of Wsi18 promoter from O. nivara under SWS condition. This is the first report of characterization of an inducible promoter from a wild rice genotype to drive the gene expression under water stress conditions. The Wsi18 promoter element from the wild rice genotype can be used in future genetic manipulation strategies for the generation of SWS tolerant rice cultivars with improved yield characteristics.