AtPLC2, a gene encoding phosphoinositide-specificphospholipase C, is constitutively expressedin vegetative and floral tissues in Arabidopsisthaliana
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- 作者：Hirayama ; Takashi ; Mitsukawa ; Norihiro ; Shibata ; Daisuke ; Shinozaki ; Kazuo
- 关键词：phosphoinositide ; specific phospholipase C ; chromosomal mapping ; Arabidopsis thaliana
- 刊名：Plant Molecular Biology
- 出版年：1997
- 出版时间：1997
- 年：1997
- 卷：34
- 期：1
- 页码：175-180
- 全文大小：220 KB
文摘
A cDNA encoding a phosphoinositide-specificphospholipase C (PI-PLC) from the higher plantArabidopsis thalianawas cloned and characterized.
The gene corresponding to this cDNA is designated AtPLC2. The overall structure of the predictedAtPLC2 protein is similar to those of plant PI-PLCsand mammalian δ-type PI-PLCs. Northern blotanalysis revealed that AtPLC2 is expressedconstitutively whereas AtPLC1S, another gene forPI-PLC of Arabidopsis, is induced byenvironmental stresses such as dehydration and salinity,indicating that the function of AtPLC2 isdistinct from that of AtPLC1S. The AtPLC2 mRNAwas detected in vegetative and floral tissues. Wedetermined the positions of these two PI-PLCs genes onArabidopsischromosomes by RFLP mapping using P1genomic clones.