Evaluation of Inhibitory Activity of Casein on Proteases in Rat Intestine

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• 作者：Seiji Ohtani ; Kyoko Shirasu ; Ken-ichi Ogawara ; Kazutaka Higaki and Toshikiro Kimura
• 关键词：casein ; trypsin ; chymotrypsin ; inhibitory activity ; small intestine
• 刊名：Pharmaceutical Research
• 出版年：2003
• 出版时间：2003
• 年：2003
• 卷：20
• 期：4
• 页码：611-617
• 全文大小：100 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Pharmacology and Toxicology
  Pharmacy
  Biochemistry
  Medical Law
  Biomedical Engineering
  
• 出版者：Springer Netherlands
• ISSN：1573-904X

文摘

Purpose. To investigate the possible use of casein as an adjuvant for oral delivery of peptide drugs, the inhibitory activity of casein on proteases in rat small intestine was examined.Methods. Male Sprague-Dawley rats weighing 200-300 g were used as the animal model. The luminal contents of the small-intestinal tract and mucosal subcellular fractions of the small intestine were prepared; the enzymatic activities of trypsin, chymotrypsin, aminopeptidase-B, leucine aminopeptidase, dipeptidylaminopeptidase-IV, cathepsin B, and dipeptidylaminopeptidase-II were determined using a specific substrate for each protease; and the effect of casein on the protease activity was examined.