Identifying and characterizing Yarrowia keelungensis sp. nov., an oil-degrading yeast isolated from the sea surface microlayer

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• 作者：Chin-Feng Chang (1)
  Chih-Chen Chen (1)
  Ching-Fu Lee (2)
  Shiu-Mei Liu (1)
  
• 关键词：Diesel oil degradation ; New species ; Yeast ; Sea surface microlayer ; UV A/B resistance
• 刊名：Antonie van Leeuwenhoek
• 出版年：2013
• 出版时间：December 2013
• 年：2013
• 卷：104
• 期：6
• 页码：1117-1123
• 全文大小：
• 作者单位：Chin-Feng Chang (1)
  Chih-Chen Chen (1)
  Ching-Fu Lee (2)
  Shiu-Mei Liu (1)
  
  1. Graduate Institute of Marine Biology, National Taiwan Ocean University, 2 Pei-Ning Road, Keelung, 20224, Taiwan
  2. Department of Applied Science, National Hsinchu University of Education, 521 Nanda Road, Hsinchu, 30014, Taiwan
  
• ISSN：1572-9699

文摘

Ascomycetous yeast strain SM-22 was isolated from the sea-surface microlayer near the Keelung City off the northern coast of Taiwan. This strain showed a cell surface hydrophobicity higher than 90?%, moderate UV A/B resistance, and it degraded 68?% of the total petroleum hydrocarbon content of an artificial seawater medium containing 1?% (v?v?1) diesel oil within 15?days at 25??. The closest phylogenetic relative of this strain is Candida oslonensis CBS 10146T, but it differs from strain SM-22 by a 3.7?% divergence (including 18 nucleotide substitutions and 2 gaps) in the D1/D2 domain sequence of the large subunit rRNA gene. This difference clearly suggests that the strain SM-22 represents a distinct species. Strain SM-22 does not produce ascospores on common sporulation media and it can therefore be considered an anamorph of the genus Yarrowia. Thus, the name Yarrowia keelungensis sp. nov. (type strain SM-22T?=?BCRC 23110T?=?JCM 14894T?=?CBS 11062T) is proposed as a novel species of genus Yarrowia.