Clinical relevance of the interleukin 10 promoter polymorphisms in Chinese Han patients with major trauma: genetic association studies

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• 作者：Ling Zeng (1)
  Wei Gu (1)
  Kehong Chen (1)
  Dongpo Jiang (2)
  Lianyang Zhang (2)
  Dingyuan Du (3)
  Ping Hu (3)
  Qing Liu (1)
  Suna Huang (1)
  Jianxin Jiang (1)
  
• 刊名：Critical Care
• 出版年：2009
• 出版时间：December 2009
• 年：2009
• 卷：13
• 期：6
• 全文大小：
• 作者单位：Ling Zeng (1)
  Wei Gu (1)
  Kehong Chen (1)
  Dongpo Jiang (2)
  Lianyang Zhang (2)
  Dingyuan Du (3)
  Ping Hu (3)
  Qing Liu (1)
  Suna Huang (1)
  Jianxin Jiang (1)
  
  1. State Key Laboratory of Trauma, Burns and Combined Injury, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Changjiang Road, Yuzhong District, 400042, Chongqing, PR, China
  2. Department of Traumatic Surgery, Daping Hospital, Third Military Medical University, Changjiang Road, Yuzhong District, 400042, Chongqing, PR, China
  3. Chongqing Emergency Medical Center, Jiankang Road, Yuzhong District, 400042, Chongqing, PR, China
  
• ISSN：1364-8535

文摘

Introduction An excessive inflammatory response is thought to account for the pathogenesis of sepsis and multiple organ dysfunction syndrome (MODS) after severe trauma. The interleukin-10 (IL-10) is a potent anti-inflammatory cytokine. The objectives of this prospective study were to investigate the distribution of IL-10 promoter polymorphisms in a cohort of 308 Chinese Han patients with major trauma, and to identify associations of IL-10 promoter polymorphisms with IL-10 production and incidence of sepsis and MODS. Methods A total of 308 patients with major trauma were included in this study. The genotypes of polymorphisms -1082, -819 and -592 were determined by polymerase chain reaction-restriction fragment length polymorphism. The IL-10 levels in the supernatants were determined with enzyme-linked immunoabsorbent assay. Results The -1082A and -592A alleles were significantly associated with lower lipopolysaccharide-induced IL-10 production in an allele-dose dependent fashion. There was no significant difference for the -819 polymorphism. Except for the -1082 polymorphism, the -819 and -592 polymorphisms were not significantly associated with sepsis morbidity rate and MOD scores. Conclusions Our results further confirm the functionality of the IL-10 promoter single nucleotide polymorphisms in relation to IL-10 production. They also suggest that individual difference in IL-10 production in trauma patients might be at least in part related to genetic variations in the IL-10 promoter region.