RT-PCR-RFLP for genetic diversity analysis of the citrus tatter leaf virus strain of Apple stem grooving virus

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• 作者：Zhen Song ; Kehong Liu ; Zhongan Li ; Changyong Zhou
• 关键词：Citrus tatter leaf virus ; Restriction fragment length polymorphisms (RFLP) ; Phylogenetic analysis
• 刊名：European Journal of Plant Pathology
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：144
• 期：3
• 页码：687-692
• 全文大小：369 KB
• 参考文献：Banerjee, N., Wang, J. Y., & Zaitlin, M. (1995). A single nucleotide change in the coat protein gene of tobacco mosaic virus is involved in the induction of severe chlorosis. Virology, 207(1), 234–239.PubMed CrossRef
  de Assis Filho, F., Paguio, O., Sherwood, J., & Deom, C. (2002). Symptom induction by Cowpea chlorotic mottle virus on Vigna unguiculata is determined by amino acid residue 151 in the coat protein. Journal of General Virology, 83(4), 879–883.PubMed CrossRef
  Fattouch, S., Acheche, H., M’hirsi, S., Mellouli, L., Bejar, S., Marrakchi, M., et al. (2005). RT-PCR-RFLP for genetic diversity analysis of Tunisian Grapevine fanleaf virus isolates in their natural host plants. Journal of Virological Methods, 127(2), 126–132.PubMed CrossRef
  Gillings, M., Broadbent, P., Indsto, J., & Lee, R. (1993). Characterisation of isolates and strains of citrus tristeza closterovirus using restriction analysis of the coat protein gene amplified by the polymerase chain reaction. Journal of Virological Methods, 44(2), 305–317.PubMed CrossRef
  He, X., Jiang, Y., & Zhao, X. (1996). Comparison of pathogenicity between 11 isolates of citrus tatter leaf virus. Journal of Yunnan Agricultural University, 8(3), 240–241.
  Komatsu, K., Hirata, H., Fukagawa, T., Yamaji, Y., Okano, Y., Ishikawa, K., et al. (2012). Infection of capilloviruses requires subgenomic RNAs whose transcription is controlled by promoter-like sequences conserved among flexiviruses. Virus Research, 167(1), 8–15.PubMed CrossRef
  Magome, H., Yoshikawa, N., & Takahashi, T. (1999). Single-strand conformation polymorphism analysis of apple stem grooving capillovirus sequence variants. Phytopathology, 89(2), 136–140.PubMed CrossRef
  Sambade, A., López, C., Rubio, L., Flores, R., Guerri, J., & Moreno, P. (2003). Polymorphism of a specific region in gene p23 of Citrus tristeza virus allows discrimination between mild and severe isolates. Archives of Virology, 148(12), 2325–2340.PubMed CrossRef
  Song, Z., Liu, K. H., Yang, F. Y., Tang, K. Z., Li, Z. A., & Zhou, C. Y. (2009). Cloning and sequence analysis of the CP gene of Citrus tatter leaf virus. Scientia Agricultura Sinica, 42(10), 3741–3748.
  Tamura, K., Stecher, G., Peterson, D., Filipski, A., & Kumar, S. (2013). MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30(12), 2725–2729.PubMed PubMedCentral CrossRef
  Tatineni, S., Afunian, M. R., Hilf, M. E., Gowda, S., Dawson, W. O., & Garnsey, S. M. (2009). Molecular characterization of Citrus tatter leaf virus historically associated with Meyer lemon trees: complete genome sequence and development of biologically active in vitro transcripts. Phytopathology, 99(4), 423–431.PubMed CrossRef
  Vives, M. C., Rubio, L., Galipienso, L., Navarro, L., Moreno, P., & Guerri, J. (2002). Low genetic variation between isolates of Citrus leaf blotch virus from different host species and of different geographical origins. Journal of General Virology, 83(10), 2587–2591.PubMed CrossRef
  XianChao, S., ChangYong, Z., Ling, Q., & ShuiYing, Y. (2009). Advances in research on citrus tatter leaf virus. Acta Horticulturae Sinica, 26(2), 213–216.
  Zhou, C., Hailstones, D., Connor, R., Barkley, P., & Bowyer, J. (2001). A micro and rapid nucleotide acid extraction method of Citrus tristeza virus for amplification by RT-PCR. Journal of Fujian Agricultural University, 30, 200.
  
• 作者单位：Zhen Song (1) (2)
  Kehong Liu (1) (2)
  Zhongan Li (1) (2)
  Changyong Zhou (1) (2)
  
  1. Citrus Research Institute of Southwest University, Chongqing, 400712, People’s Republic of China
  2. National Engineering Research Center for Citrus, Chongqing, 400712, People’s Republic of China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Pathology
  Plant Sciences
  Ecology
  
• 出版者：Springer Netherlands
• ISSN：1573-8469

文摘

A method for detecting genetic diversity of the Citrus tatter leaf virus (CTLV) strain of Apple stem grooving virus was developed based on restriction fragment length polymorphisms (RFLP) of the 889 nt 3′ sequence amplified by RT-PCR, and nine Hinf I RFLP patterns were defined. This RFLP assay, together with biological indexing and sequencing, was applied to characterize 18 CTLV isolates collected from seven provinces of China. The results indicated that the majority of these isolates (16/18) presented as single well-defined RFLP patterns, among which RFLPIand RFLPII were dominant. In contrast, two samples exhibited as a mixture of two RFLP patterns, suggesting a mixed infection of CTLV variants. Of the 18 isolates, six of eight samples with RFLPIIor RFLP III induced mild to moderate symptoms on indexing plants (Citrus. sinensis × Poncirus trifoliata cv. Rusk), while four isolates conforming to RFLPIand six samples to RFLP IV ~ IX induced severe symptoms. In the phylogenetic tree constructed according to the 3′ nucleotide sequence of the 18 CTLV isolates, RFLPIIand RFLP III samples clustered into phylogenetic group A, whereas the others gathered into phylogenetic group B. These results revealed correlation among mild (moderate) symptoms, phylogenetic group A and RFLPIIor RFLP III restrictotypes, suggesting the RFLP assay may be useful in quick identification of CTLV strains. Keywords Citrus tatter leaf virus Restriction fragment length polymorphisms (RFLP) Phylogenetic analysis