Application of a quantitative ¹H-NMR method for the determination of paeonol in Moutan cortex, Hachimijiogan and Keishibukuryogan

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• 作者：Rie Tanaka ; Hikari Shibata ; Naoki Sugimoto ; Hiroshi Akiyama…
• 关键词：Paeonol ; Quantitative 1H ; NMR ; Moutan cortex ; Hachimijiogan ; Keishibukuryogan ; Reagent purity
• 刊名：Journal of Natural Medicines
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：70
• 期：4
• 页码：797-802
• 全文大小：516 KB
• 刊物主题：Pharmacology/Toxicology; Plant Sciences; Complementary & Alternative Medicine; Medicinal Chemistry; Pharmacy;
• 出版者：Springer Japan
• ISSN：1861-0293
• 卷排序：70

文摘

Quantitative 1H-NMR (1H-qNMR) was applied to the determination of paeonol concentration in Moutan cortex, Hachimijiogan, and Keishibukuryogan. Paeonol is a major component of Moutan cortex, and its purity was calculated from the ratio of the intensity of the paeonol H-3′ signal at δ 6.41 ppm in methanol-d₄ or 6.40 ppm in methanol-d₄ + TFA-d to that of a hexamethyldisilane (HMD) signal at 0 ppm. The concentration of HMD was corrected with SI traceability by using potassium hydrogen phthalate of certified reference material grade. As a result, the paeonol content in two lots of Moutan cortex as determined by 1H-qNMR was found to be 1.59 % and 1.62 %, respectively, while the paeonol content in Hachimijiogan and Keishibukuryogan was 0.15 % and 0.22 %, respectively. The present study demonstrated that the 1H-NMR method is useful for the quantitative analysis of crude drugs and Kampo formulas.