Decreased expression of key tumour suppressor microRNAs is associated with lymph node metastases in triple negative breast cancer

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• 作者：Kelly A Avery-Kiejda (1) (2)
  Stephen G Braye (3)
  Andrea Mathe (1) (2)
  John F Forbes (4) (5)
  Rodney J Scott (1) (2) (3)
  
• 关键词：miRNA ; Breast cancer ; Triple negative ; Metastases ; Tumour suppressor
• 刊名：BMC Cancer
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：14
• 期：1
• 全文大小：714 KB
• 作者单位：Kelly A Avery-Kiejda (1) (2)
  Stephen G Braye (3)
  Andrea Mathe (1) (2)
  John F Forbes (4) (5)
  Rodney J Scott (1) (2) (3)
  
  1. Centre for Information-Based Medicine, Hunter Medical Research Institute, New Lambton Heights, NSW, 2305, Australia
  2. School of Biomedical Sciences and Pharmacy, Faculty of Health, University of Newcastle, Callaghan, NSW, 2308, Australia
  3. Hunter Area Pathology Service, John Hunter Hospital, New Lambton Heights, NSW, 2305, Australia
  4. Department of Surgical Oncology, Calvary Mater Newcastle Hospital, Australian New Zealand Breast Cancer Trials Group, Waratah, NSW, 2298, Australia
  5. School of Medicine and Public Health, Faculty of Health, University of Newcastle, Callaghan, NSW, 2308, Australia
  
• ISSN：1471-2407

文摘

Background Breast cancer is the most common malignancy that develops in women, responsible for the highest cancer-associated death rates. Triple negative breast cancers represent an important subtype that have an aggressive clinical phenotype, are associated with a higher likelihood of metastasis and are not responsive to current targeted therapies. miRNAs have emerged as an attractive candidate for molecular biomarkers and treatment targets in breast cancer, but their role in the progression of triple negative breast cancer remains largely unexplored. Methods This study has investigated miRNA expression profiles in 31 primary triple negative breast cancer cases and in 13 matched lymph node metastases compared with 23 matched normal breast tissues to determine miRNAs associated with the initiation of this disease subtype and those associated with its metastasis. Results 71 miRNAs were differentially expressed in triple negative breast cancer, the majority of which have previously been associated with breast cancer, including members of the miR-200 family and the miR-17-92 oncogenic cluster, suggesting that the majority of miRNAs involved in the initiation of triple negative breast cancer are not subtype specific. However, the repertoire of miRNAs expressed in lymph node negative and lymph node positive triple negative breast cancers were largely distinct from one another. In particular, miRNA profiles associated with lymph node negative disease tended to be up-regulated, while those associated with lymph node positive disease were down-regulated and largely overlapped with the profiles of their matched lymph node metastases. From this, 27 miRNAs were identified that are associated with metastatic capability in the triple negative breast cancer subtype. Conclusions These results provide novel insight into the repertoire of miRNAs that contribute to the initiation of and progression to lymph node metastasis in triple negative breast cancer and have important implications for the treatment of this breast cancer subtype.