The preliminary performance study of the 3D printing of a tricalcium phosphate scaffold for the loading of sustained release anti-tuberculosis drugs

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• 作者：Jing Yuan ; Ping Zhen ; Hongbin Zhao ; Keming Chen ; Xusheng Li…
• 刊名：Journal of Materials Science
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：50
• 期：5
• 页码：2138-2147
• 全文大小：2,577 KB
• 参考文献：1. Pigrau-Serrallach C, Rodríguez-Pardo D (2013) Bone and joint tuberculosis. Eur Spine J 22(4):556-66 CrossRef
  2. Ye X, Zhen P, Li X et al (2010) Study on preparation and properties of novel anti-TB porous calcium phosphate cement. Orthop J Chin 18(23):1981-986
  3. Gu Y, Chen X, Lee J-H et al (2012) Inkjet printed antibiotic-and calcium-eluting bioresorbable nanocomposite micropatterns for orthopedic implants. Acta Biomater 8(1):424-31 CrossRef
  4. Wu W, Zheng Q, Guo X (2010) Experimental Study of a Rifampicine-isoniazid-controlled Release Drug Implant. Chin J Biomed Eng 1:137-43
  5. Liu P, Jiang J (2011) High performance liquid chromatagraphy to test rifampin and desacetylrifampin in spinal tuberculosis. J Chin Med Univ 40(7):665-68
  6. Li D, Ma Y (2013) Research progresses of drug delivery material in the treatment of osteoaticular tuberculosis. Chin J Antituberc 35(5):25-2
  7. Chen Q (2013) Study on the Toxicity of Isoniazide with Various Concentrations on Cultured Osteoblasts in vitro[D]. huazhong university of science and technology 31(3):21-7
  8. Guo D, Wu H, Xue G et al (2011) The hepatocyet toxicity and the effect of Isoniazid and Rifampicin on the activities of CYP2E1 and CYP3A in mouse primary hepatocytes. Pham J Chin PLA 27(3):221-23
  9. Mao K, Yang Y, Li J et al (2009) Investigation of the histology and interfacial bonding between carbonated hydroxyapatite cement and bone. Biomed Mater 4(4):045003 CrossRef
  10. Rihn JA, Kirkpatrick K, Albert TJ (2010) Graft options in posterolateral and posterior interbody lumbar fusion. Spine 35(17):1629-639 CrossRef
  11. Schroeder JE, Mosheiff (2011) Tissue engineering approaches for bone repair: concepts and evidence. Injury 42(6):609-13 CrossRef
  12. Sugawara A, Asaoka K, Ding SJ (2013) Calcium phosphate-based cements: clinical needs and recent progress. J Mater Chem B 1(8):1081-089 CrossRef
  13. Dorozhkin SV (2010) Bioceramics of calcium orthophosphates. Biomaterials 31(7):1465-485 CrossRef
  14. Wu C, Chang J (2013) A review of bioactive silicate ceramics. Biomed Mater 8(3):032001 CrossRef
  15. Nischang I, Teasdale I, Brüggemann O (2011) Porous polymer monoliths for small molecule separations: advancements and limitations. Anal Bioanal Chem 400(8):2289-304 CrossRef
  16. Lu L, Zhang Q, Wootton D et al (2012) Biocompatibility and biodegradation studies of PCL/β-TCP bone tissue scaffold fabricated by structural porogen method. J Mater Sci 23(9):2217-226
  17. Butscher A, Bohner M, Hofmann S et al (2011) Structural and material approaches to bone tissue engineering in powder-based three-dimensional printing. Acta Biomater 7(3):907-20 CrossRef
  18. Wei X, Zhao N, Lin Z et al (2013) Preparation and characterization of three-dimensional porus β-tricalcium phosphate scaffold with controlled porous. J Funct Mater 43(23):3217-221
  19. Bao Y, Zhang W, Wang Y et al (2012) Preparation and release features of long-term slow-release two-component drug artificial bone. Chin J Tissue Eng Res 16(38):7126-130
  20. Karageorgiou V, Kaplan D (2005) Porosity of 3D biomaterial scaffolds and osteogenesis. Biomaterials 26(27):5474-491 CrossRef
  21. Miranda P, Pajares A, Saiz E et al (2007) Fracture modes under uniaxial compression in hydroxyapatite scaffolds fabricated by robocasting. J Biomed Mater Res Part A 83(3):646-55 CrossRef
  22. Miranda P, Pajares A, Guiberteau F (2008) Finite element modeling as a tool for predict
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Materials Science
  Characterization and Evaluation Materials
  Polymer Sciences
  Continuum Mechanics and Mechanics of Materials
  Crystallography
  Mechanics
  
• 出版者：Springer Netherlands
• ISSN：1573-4803

文摘

In the surgical treatment of tuberculosis of the bones, excision of the lesion site leaves defects in the bone structure. Recent research has shown benefits for bone tissue support, such as tricalcium phosphate, as regrowth materials. These biocompatible engineering materials have good bone inductivity and biologic mechanical performance. The goal of this study was to evaluate the use of 3D printing, a new technology, to design and build 3-dimensional support structures for use in grafting at lesion sites and for use in embedding the sustained release anti-tuberculosis drugs Rifampin and Isoniazid and determine the in vivo performance of these structures. In addition to mechanical studies, osteogenesis, cell viability, and migration were all observed, using Wistar rat models, to determine the effectiveness of this material as a biological support. The bone support showed good resistance to compression, similar to the spongiest bone tissue, and high porosity. In vivo studies showed that the material had a stable time release of Rifampin and Isoniazid through 90?days and achieved effective killing of the tuberculosis-causing bacteria. Finally, the support allowed for good migration and survival of rat bone marrow mesenchymal stem cells, leading to successful bone regrowth and repair. These results imply that the use of 3D printing of tricalcium phosphate scaffolds for bone excision repair and time-release treatment of tuberculosis shows great promise for future treatment of patients with tuberculosis of the bones.