A simple method for the production of bacterial controls for immunohistochemistry and fluorescent in situ hybridization

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• 作者：Camilla Recordati ; Enrico Radaelli ; Kenneth W. Simpson and Eugenio Scanziani
• 关键词：Immunohistochemistry ; Fluorescent in situ hybridization ; Bacteria ; Controls
• 刊名：Journal of Molecular Histology
• 出版年：2008
• 出版时间：August, 2008
• 年：2008
• 卷：39
• 期：4
• 页码：459-462
• 全文大小：240.3 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Cell Biology
  Biomedicine
  Developmental Biology
  
• 出版者：Springer Netherlands
• ISSN：1567-2387

文摘

Immunohistochemical and fluorescent in situ hybridization (FISH) assays are useful diagnostic methods for the identification of bacteria on formalin fixed paraffin embedded histological sections. To validate an anti-bacterial antibody or an oligonucleotide probe and to ensure fidelity during subsequent analyses, suitable positive and negative controls are necessary. Suspensions of fixed bacteria are often used, but ideally, these controls should be fixed, embedded and processed in the same way of tissue samples under analysis. Herein, we describe a simple method for the production of bacterial histological control samples: the sandwich. The sandwich is composed of two external layers of equine lung parenchyma and a central layer of the target bacterium. We prepared sandwiches containing Escherichia coli, Campylobacter jejuni, and Arcanobacterium pyogenes and tested them with appropriate antibodies and Eub338 FISH probe. The sandwich is an effective and simple method to prepare bacterial histological controls fixed and processed in the same way as the diagnostic tissues.