Proteomic analysis of enterotoxigenic ss="a-plus-plus">Escherichia coli (ETEC) in neutral and alkaline conditions
详细信息 查看全文
- 作者：Lucia Gonzales-Siles ; Roger Karlsson ; Diarmuid Kenny ; Anders Karlsson…
- 关键词：ETEC ; pH regulation ; Proteomics ; Alkaline ; ATPase ; OmpA ; BAM
- 刊名：BMC Microbiology
- 出版年：2017
- 出版时间：December 2017
- 年：2017
- 卷：17
- 期：1
- 全文大小：1448KB
- 刊物主题：Microbiology; Biological Microscopy; Mycology; Parasitology; Virology; Life Sciences, general;
- 出版者：BioMed Central
- ISSN：1471-2180
- 卷排序：17
文摘
BackgroundEnterotoxigenic Escherichia coli (ETEC) is a major cause of diarrhea in children and travelers to endemic areas. Secretion of the heat labile AB<sub>5sub> toxin (LT) is induced by alkaline conditions. In this study, we determined the surface proteome of ETEC exposed to alkaline conditions (pH 9) as compared to neutral conditions (pH 7) using a LPI Hexalane FlowCell combined with quantitative proteomics. Relative quantitation with isobaric labeling (TMT) was used to compare peptide abundance and their corresponding proteins in multiple samples at MS/MS level. For protein identification and quantification samples were analyzed using either a 1D-LCMS or a 2D-LCMS approach.