Etiological Analysis of Fungal Keratitis and Rapid Identification of Predominant Fungal Pathogens

详细信息    查看全文

• 作者：Dan He ; Jilong Hao ; Song Gao ; Xue Wan ; Wanting Wang ; Qiushi Shan ; Li Wang
• 关键词：Fungi ; Keratitis ; Epidemiology ; Identification ; Multiplex PCR
• 刊名：Mycopathologia
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：181
• 期：1-2
• 页码：75-82
• 全文大小：708 KB
• 参考文献：1.Vengayil S, Panda A, Satpathy G, et al. Polymerase chain reaction-guided diagnosis of mycotic keratitis: a prospective evaluation of its efficacy and limitations. Invest Ophthalmol Vis Sci. 2009;50:152鈥?.CrossRef PubMed
  2.Thomas PA, Kaliamurthy J. Mycotic keratitis: epidemiology, diagnosis, and management. Clin Microbiol Infect. 2013;19:210鈥?0.CrossRef PubMed
  3.Shukla PK, Kumar M, Keshava GB. Mycotic keratitis: an overview of diagnosis and therapy. Mycoses. 2008;51:183鈥?9.CrossRef PubMed
  4.Thomas PA. Current perspectives on ophthalmic mycoses. Clin Microbiol Rev. 2003;16:730鈥?7.PubMedCentral CrossRef PubMed
  5.Thomas PA. Fungal infections of the cornea. Eye (Lond). 2003;17:852鈥?2.CrossRef
  6.Sirikul T, Prabriputaloong T, Smathivat A, Chuck RS, Vongthongsri A. Predisposing factors and etiologic diagnosis of ulcerative keratitis. Cornea. 2008;27:283鈥?.CrossRef PubMed
  7.He D, Hao J, Zhang B, et al. Pathogenic spectrum of fungal keratitis and specific identification of Fusarium solani. Invest Ophthalmol Vis Sci. 2011;52:2804鈥?.CrossRef PubMed
  8.Wang L, Yokoyama K, Miyaji M, Nishimura K. Mitochondrial cytochrome b gene analysis of Aspergillus fumigatus and related species. J Clin Microbiol. 2000;38:1352鈥?.PubMedCentral PubMed
  9.Kalkanci A, Ozdek S. Ocular fungal infections. Curr Eye Res. 2011;36:179鈥?9.CrossRef PubMed
  10.Shah A, Sachdev A, Coggon D, Hossain P. Geographic variations in microbial keratitis: an analysis of the peer-reviewed literature. Br J Ophthalmol. 2011;95:762鈥?.PubMedCentral CrossRef PubMed
  11.Tu EY, McCartney DL, Beatty RF, Springer KL, Levy J, Edward D. Successful treatment of resistant ocular fusariosis with posaconazole (SCH-56592). Am J Ophthalmol. 2007;143:222鈥?.CrossRef PubMed
  12.Chowdhary A, Singh K. Spectrum of fungal keratitis in North India. Cornea. 2005;24:8鈥?5.CrossRef PubMed
  13.Galarreta DJ, Tuft SJ, Ramsay A, Dart JK. Fungal keratitis in London: microbiological and clinical evaluation. Cornea. 2007;26:1082鈥?.CrossRef PubMed
  14.Gower EW, Keay LJ, Oechsler RA, et al. Trends in fungal keratitis in the United States, 2001 to 2007. Ophthalmology. 2010;117:2263鈥?.CrossRef PubMed
  15.Zhong WX, Sun SY, Zhao J, Shi WY, Xie LX. Retrospective study of suppurative keratitis in 1054 patients. Zhonghua Yan Ke Za Zhi. 2007;43:245鈥?0.PubMed
  16.Xie L, Zhong W, Shi W, Sun S. Spectrum of fungal keratitis in north China. Ophthalmology. 2006;113:1943鈥?.CrossRef PubMed
  17.Shi W, Wang T, Xie L, et al. Risk factors, clinical features, and outcomes of recurrent fungal keratitis after corneal transplantation. Ophthalmology. 2010;117:890鈥?.CrossRef PubMed
  18.Tuft SJ, Tullo AB. Fungal keratitis in the United Kingdom 2003鈥?005. Eye (Lond). 2009;23:1308鈥?3.CrossRef
  19.Sengupta J, Khetan A, Saha S, Banerjee D, Gangopadhyay N, Pal D. Candida keratitis: emerging problem in India. Cornea. 2012;31:371鈥?.CrossRef PubMed
  20.Chakrabarti A, Singh R. The emerging epidemiology of mould infections in developing countries. Curr Opin Infect Dis. 2011;24:521鈥?.CrossRef PubMed
  21.Ghosh A, Basu S, Datta H, Chattopadhyay D. Evaluation of polymerase chain reaction-based ribosomal DNA sequencing technique for the diagnosis of mycotic keratitis. Am J Ophthalmol. 2007;144:396鈥?03.CrossRef PubMed
  22.Bu R, Sathiapalan RK, Ibrahim MM, et al. Monochrome Light Cycler PCR assay for detection and quantification of five common species of Candida and Aspergillus. J Med Microbiol. 2005;54:243鈥?.CrossRef PubMed
  23.Esposto MC, Cogliati M, Tortorano AM, Viviani MA. Determination of Cryptococcus neoformans var. neoformans mating type by multiplex PCR. Clin Microbiol Infect. 2004;10:1092鈥?.CrossRef PubMed
  
• 作者单位：Dan He (1)
  Jilong Hao (2)
  Song Gao (1)
  Xue Wan (1)
  Wanting Wang (1)
  Qiushi Shan (1)
  Li Wang (1)
  
  1. Department of Pathogenobiology, Jilin University Mycology Research Center, Key Laboratory of Pathobiology, Ministry of Education, College of Basic Medical Sciences, Jilin University, No. 126 Xinmin Street, Changchun City, Jilin Province, 130021, China
  2. Department of Ophthalmology, First Hospital of Jilin University, Changchun City, 130021, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Medical Microbiology
  Plant Sciences
  Microbial Ecology
  
• 出版者：Springer Netherlands
• ISSN：1573-0832

文摘

Fungal keratitis is a worldwide-distributed refractory and potentially blinding ocular infection caused by various fungi. It is necessary to investigate the etiological and epidemiological characteristics of this disease and establish a rapid and specific pathogenic identification method. Here, we isolated and identified fungal pathogens of 275 patients with presumed fungal keratitis from Jilin Province, China, and conducted statistical analyses of epidemiological information. The positive rate of fungal culture was 72.0 %. Fusarium sp. was the most common genus among 210 fungal isolates. The predominant species were Fusarium solani, Aspergillus fumigatus, and Candida glabrata, which accounted for over 50 % of the isolated organisms. Corneal trauma and previous use of drugs were the most important predisposing factors. In addition, a multiplex polymerase chain reaction (PCR) was designed with species-specific primers of the three species that could identify them with amplicons of approximately 330 bp from F. solani, 275 bp from A. fumigatus, and 230 bp from C. glabrata. Additionally, PCR with fungal universal primers and multiplex PCR were performed using DNA prepared by an improved DNA extraction method from corneal scrapings. With this method, fungal pathogens from corneal scrapings could be specifically and rapidly identified within 8 h. The culture-independent rapid identification of corneal scrapings may have great significance for the early diagnosis and treatment of fungal keratitis. Keywords Fungi Keratitis Epidemiology Identification Multiplex PCR