Trans-Caryophyllene Suppresses Hypoxia-Induced Neuroinflammatory Responses by Inhibiting NF-κB Activation in Microglia

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• 作者：Kexin Guo (1)
  Xiaojie Mou (1)
  Jinsha Huang (1)
  Nian Xiong (1)
  Hongge Li (1) (2)
  
• 关键词：Trans ; Caryophyllene ; Hypoxia ; NF ; κB ; Type 2 cannabinoid receptor (CB2R) ; Inflammation
• 刊名：Journal of Molecular Neuroscience
• 出版年：2014
• 出版时间：September 2014
• 年：2014
• 卷：54
• 期：1
• 页码：41-48
• 全文大小：1,449 KB
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• 作者单位：Kexin Guo (1)
  Xiaojie Mou (1)
  Jinsha Huang (1)
  Nian Xiong (1)
  Hongge Li (1) (2)
  
  1. Department of Neurology, Wuhan Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
  2. #1277 Jiefang Avenue, Wuhan, Hubei, 430022, China
  
• ISSN：1559-1166

文摘

Microglia cells have been reported to mediate hypoxia-induced inflammation through the production of proinflammatory cytokines, including interleukin-1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), and IL-6. Given the fact that the activation of the type 2 cannabinoid receptor (CB2R) provides antioxidative and anti-inflammatory results, it is suspected that its selective agonist, trans-caryophyllene (TC), may have protective effects against hypoxia-induced neuroinflammatory responses. In this study, TC was found to significantly inhibit hypoxia-induced cytotoxicity as well as the release of proinflammatory cytokines, including IL-1β, TNF-α, and IL-6, through activation of BV2 microglia following hypoxic exposure (1?% O2, 24?h). Furthermore, TC significantly inhibited hypoxia-induced generation of reactive oxygen species (ROS) in mitochondria as well as the activation of nuclear factor kappa B (NF-κB) in microglia. Importantly, TC’s effects on inhibiting the activation of NF-κB and the secretion of inflammatory cytokines can be abolished by muting the CB2R using small RNA interference. These observations indicate that TC suppresses the hypoxia-induced neuroinflammatory response through inhibition of NF-κB activation in microglia. Therefore, TC may be beneficial in preventing hypoxia-induced neuroinflammation.