Design of a stable cell line producing recombinant darbepoetin alpha based on CHO cells

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• 作者：R. R. Shukurov (1)
  N. V. Lobanova (1)
  I. N. Savinova (1)
  I. G. Vorobyova (1)
  A. A. Nurbakov (1)
  L. V. Ermolina (2)
  N. V. Orlova (1)
  A. G. Mosina (1)
  L. P. Antonova (1)
  R. A. Khamitov (2)
  Yu. A. Seryogin (1)
  
• 关键词：cell line ; CHO cells ; darbepoetin ; erythropoetin ; expression ; transfection
• 刊名：Applied Biochemistry and Microbiology
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：50
• 期：9
• 页码：812-818
• 全文大小：481 KB
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• 作者单位：R. R. Shukurov (1)
  N. V. Lobanova (1)
  I. N. Savinova (1)
  I. G. Vorobyova (1)
  A. A. Nurbakov (1)
  L. V. Ermolina (2)
  N. V. Orlova (1)
  A. G. Mosina (1)
  L. P. Antonova (1)
  R. A. Khamitov (2)
  Yu. A. Seryogin (1)
  
  1. Limited Liability Company 鈥淧HARMAPARK,鈥? Moscow, 117246, Russia
  2. The State Research Institute for Genetics and Selection of Industrial Microorganisms, Moscow, 117545, Russia
  
• ISSN：1608-3024

文摘

A stable cell line that is based on CHO cells and produces 100 mg per liter of culture medium of recombinant darbepoetin alpha with a target glycosylated isoforms effective yield of about 30% has been selected. The expression product of the cell line was characterized and compared to the originator (Aranesp, Amgen). It was shown that the obtained preparation contained all isoforms characteristic of the originator. The created cell line can be used for the development of industrial cultivation and a purification scheme for recombinant darbepoetin alpha production.