Rapid, cost-effective DNA quantification via a visually-detectable aggregation of superparamagnetic silica-magnetite nanoparticles
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- 作者：Qian Liu (1) (4)
Jingyi Li (1) (4)
Hongxue Liu (5)
Ibrahim Tora (1)
Matthew S. Ide (6)
Jiwei Lu (5)
Robert J. Davis (6)
David L. Green (6)
James P. Landers (1) (2) (3) (4) - 关键词：silica/magnetite ; core ; shell ; superparamagnetic ; DNA quantification ; polymerase chain reaction ; (PCR)
- 刊名：Nano Research
- 出版年：2014
- 出版时间：May 2014
- 年：2014
- 卷：7
- 期：5
- 页码：755-764
- 全文大小：
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Jingyi Li (1) (4)
Hongxue Liu (5)
Ibrahim Tora (1)
Matthew S. Ide (6)
Jiwei Lu (5)
Robert J. Davis (6)
David L. Green (6)
James P. Landers (1) (2) (3) (4)
1. Department of Chemistry, University of Virginia, McCormick Road, P. O. Box 400319, Charlottesville, Virginia, 22904, USA
4. Center for Microsystems for the Life Sciences, University of Virginia, Charlottesville, Virginia, 22904, USA
5. Department of Materials Science & Engineering, University of Virginia, P. O. Box 400745, 395 McCormick Road, Charlottesville, Virginia, 22904-4745, USA
6. Department of Chemical Engineering, University of Virginia, 123 Engineers-Way, Charlottesville, Virginia, 22904, USA
2. Department of Pathology, University of Virginia Health Science Center, Charlottesville, Virginia, 22908, USA
3. Department of Mechanical Engineering, University of Virginia, Charlottesville, Virginia, 22904, USA - ISSN：1998-0000
文摘
DNA and silica-coated magnetic particles entangle and form visible aggregates under chaotropic conditions with a rotating magnetic field, in a manner that enables quantification of DNA by image analysis. As a means of exploring the mechanism of this DNA quantitation assay, nanoscale SiO2-coated Fe3O4 (Fe3O4@SiO2) particles are synthesized via a solvothermal method. Characterization of the particles defines them to be ?00 nm in diameter with a large surface area (141.89 m2/g), possessing superparamagnetic properties and exhibiting high saturation magnetization (38 emu/g). The synthesized Fe3O4@SiO2 nanoparticles are exploited in the DNA quantification assay and, as predicted, the nanoparticles provide better sensitivity than commercial microscale Dynabeads? for quantifying DNA, with a detection limit of 4 kilobase-pair fragments of human DNA. Their utility is proven using nanoparticle DNA quantification to guide efficient polymerase chain reaction (PCR) amplification of short tandem repeat loci for human identification.