Heritability and Genetics of Serum Dickkopf 1 Levels in African Ancestry Families

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• 作者：Allison L. Kuipers ; Shibing Yu ; Candace M. Kammerer…
• 关键词：Dickkopf ; 1 ; Heritability ; Single nucleotide polymorphism ; Gene expression ; African ancestry
• 刊名：Calcified Tissue International
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：96
• 期：2
• 页码：155-159
• 全文大小：197 KB
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• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Biochemistry
  Endocrinology
  Orthopedics
  Cell Biology
  
• 出版者：Springer New York
• ISSN：1432-0827

文摘

The aim of the study was to determine the heritability of serum dickkopf-1 (DKK1) and its association with DKK1 polymorphisms in African ancestry subjects. Serum DKK1 was measured in 422 Afro–Caribbean men and women aged 18+ from 7 large, multi-generational families (mean family size: 60; 3,215 relative pairs). Twenty-four common single nucleotide polymorphisms (SNPs) were genotyped within an 80 kilobase-pair region encompassing the DKK1 gene. Heritability was estimated and SNPs were tested for association with serum DKK1 using variance components analysis. DKK1 mRNA expression was tested in peripheral blood of 16 individuals from each of the rs7069912 genotypes. Mean serum DKK1 was 1724.1?pg/mL and was significantly lower in women than men (P?=?0.043). Residual genetic heritability of serum DKK1 was 0.4460 (P?SNPs reached nominal significance with DKK1, with rs7069912 being significant after adjustment for multiple comparisons. Two of these six SNPs represented independent association signals (rs7069912 and rs16928725), which accounted for 4.6?% of the phenotypic variation in DKK1. Additionally, carriers of the rs7069912 variant had significantly greater DKK1 expression than non-carriers (P?=?0.036). Serum DKK1 levels are highly heritable in the African ancestry families. Two SNPs within the DKK1 region accounted for nearly 5?% of the variation in serum DKK1.