Generation of chemically induced mutations using in vitro propagated shoot tip tissues for genetic improvement of fruit trees

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• 作者：Mohamed Elhiti ; Huaiyu Wang ; Ryan S. Austin…
• 关键词：Mutagenesis ; Ethyl methanesulfonate ; Peach ; In vitro multiplication ; Next generation sequencing
• 刊名：Plant Cell, Tissue and Organ Culture
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：124
• 期：2
• 页码：447-452
• 全文大小：2,993 KB
• 参考文献：Byrne D, Raseira M, Bassi D, Piagnani M, Gasic K (2012) Peach. In: Badenes ML, Byrne DH (eds) Fruit breeding. Springer, New York, pp 505–569CrossRef
  Carrasco B, Meisel L, Gebauer M, Garcia-Gonzales R, Silva H (2013) Breeding in peach, cherry and plum: from a tissue culture, genetic, transcriptomic and genomic perspective. Biol Res 46:219–230CrossRef PubMed
  Cooper J, Till BJ, Laport RG, Darlow MC, Kleffner JM et al (2008) TILLING to detect induced mutations in soybean. BMC Plant Biol 8:9PubMedCentral CrossRef PubMed
  Li H, Durbin R (2009) Fast and accurate short read alignment with Burrows–Wheeler transform. Bioinformatics 25(14):1754–1760PubMedCentral CrossRef PubMed
  Li H, Handsaker B, Wysoker A, Fennell T, Ruan J et al (2009) The sequence alignment/map format and SAMtools. Bioinformatics 25(16):2078–2079PubMedCentral CrossRef PubMed
  Moran NA, McLaughlin HJ, Sorek R (2009) The dynamics and time scale of ongoing genomic erosion in symbiotic bacteria. Science 323:37–382CrossRef
  San B, Li Z, Hu Q, Reighard GL, Luo H (2015) Adventitious shoot regeneration from in vitro cultured leaf explants of peach rootstock Guardian® is significantly enhanced by silver thiosulfate. Plant Cell Tissue Organ Cult 120:757–765CrossRef
  Sciuchetti LA, Born AE (1965) Effect of dimethylsulfoxide alone and combined with N-dimethylaminosuccinamic acid (B995) or (2-chloroethyl) trimethylammonium chloride (CCC) on the growth and alkaloid biosynthesis of Datum tatula. J Pharm Sci 64:285–289CrossRef
  Sikora P, Chawade A, Larsson M, Olsson J, Olsson O (2011) Mutagenesis as a tool in plant genetics, functional genomics, and breeding. Int J Plant Genom article# 314829
  Smith DR, Quinlan AR, Peckham HE, Makowsky K, Tao W et al (2008) Rapid whole-genome mutational profiling using next-generation sequencing technologies. Genome Res 18:1638–1642PubMedCentral CrossRef PubMed
  Talebi AB, Talebi AB, Shahrokhifar B (2012) Ethyl methane sulphonate (EMS) induced mutagenesis in Malaysian rice (cv. MR219) for lethal dose determination. Am J Plant Sci 3:1661–1665CrossRef
  Till BJ, Cooper J, Tai TH, Colowit P, Greene EA, Henikoff S, Comai L (2007) Discovery of chemically induced mutations in rice by TILLING. BMC Plant Biol 7:19PubMedCentral CrossRef PubMed
  Verde I, Abbott AG, Scalabrin S, Jung S, Shu S et al (2013) The high-quality draft genome of peach (Prunus persica) identifies unique patterns of genetic diversity, domestication and genome evolution. Nat Genet 24:1–10
  
• 作者单位：Mohamed Elhiti (1) (2)
  Huaiyu Wang (1)
  Ryan S. Austin (1)
  Bin Chen (1)
  Dan Brown (1) (3)
  Aiming Wang (1)
  
  1. Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada, 1391 Sandford Street, London, ON, N5V 4T3, Canada
  2. Department of Botany, Faculty of Science, Tanta University, Tanta, 31527, Egypt
  3. Canadian Centre for Agri-Food Research in Health and Medicine, 351 Tache Avenue, Winnipeg, MB, R2H 2A6, Canada
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  Plant Physiology
  
• 出版者：Springer Netherlands
• ISSN：1573-5044

文摘

Mutagenesis via treatment of seeds with chemical mutagens such as ethyl methanesulfonate (EMS) has been widely used for crop improvement. However, this approach is not suitable for some crop species such as clonally propagated crops and allogamous perennial plants characteristically with high levels of genome heterozygosity and a long life cycle. Here, we report direct treatment of in vitro-induced peach shoot tip tissues with EMS for generation of mutations in peach, a perennial, woody fruit tree. We optimized EMS dosage and exposure time and found that treatment of the explants with 0.2 % EMS for 16 h was optimal for generation of genetic variations. So far we have generated nearly 2000 peach seedlings. Typical EMS-induced phenotypic variations were present in the seedlings. Next generation sequencing and subsequent data analyses were performed to monitor the efficiency of mutagenesis. The mutation rate was estimated to be one mutation per 150 kilobase pairs in the mutagenized population, suggesting effectiveness of this method in inducing mutagenesis in peach. Taken together, our data open an avenue for the generation of mutant populations suitable for crop improvement in allogamous perennial plants including fruit trees and clonally propagated plants.