Isolation and characterization of microsatellites in Primula glaucescens Moretti and their cross-species amplification in P. spectabilis Tratt.
详细信息 查看全文
- 作者：Davide Perini ; Marilena Meloni ; Kirsten Wolff and Giorgio Binelli
- 关键词：Conservation and repopulation ; Microsatellite enriched library ; Hexaploid alpine plant species ; Primula glaucescens Moretti ; Primula spectabilis Tratt.
- 刊名：Conservation Genetics
- 出版年：2007
- 出版时间：September, 2007
- 年：2007
- 卷：8
- 期：5
- 页码：1263-1266
- 全文大小：136 KB
文摘
The hexaploid herbaceous species Primula glaucescens Moretti and Primula spectabilis Tratt. (2n = 6x = 66) are two endemics of the Italian Pre-Alps protected by national and international laws. In order to plan conservation strategies for natural populations and to study the influence of the latest glaciation on them we developed a set of microsatellites markers for the endangered Primula species: ten primer pairs allowed analysis of polymorphic disomic loci in P. glaucescens samples and seven of them also amplified polymorphic disomic markers in P. spectabilis.