Treatment with low-temperature atmospheric pressure plasma enhances cutaneous delivery of epidermal growth factor by regulating E-cadherin-mediated cell junctions
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- 作者：Jeong-Hae Choi (1) (2)
Seoul-Hee Nam (1)
Yeon-Suk Song (2)
Hyun-Wook Lee (3)
Hae-June Lee (4)
Kiwon Song (5)
Jin-Woo Hong (2)
Gyoo-Cheon Kim (1) - 关键词：Low temperature atmospheric pressure plasma ; Skin barrier function ; Transdermal drug delivery ; E ; cadherin ; Epidermal growth factor
- 刊名：Archives of Dermatological Research
- 出版年：2014
- 出版时间：September 2014
- 年：2014
- 卷：306
- 期：7
- 页码：635-643
- 全文大小：3,220 KB
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Seoul-Hee Nam (1)
Yeon-Suk Song (2)
Hyun-Wook Lee (3)
Hae-June Lee (4)
Kiwon Song (5)
Jin-Woo Hong (2)
Gyoo-Cheon Kim (1)
1. Department of Oral Anatomy, School of Dentistry, Yangsan Campus of Pusan National University, Beomeo-ri, Mulgeum-eup, Yangsan-si, Yangsan, Gyeongsangnam, 626-870, South Korea
2. Department of Internal Medicine, School of Korean Medicine, Yangsan Campus of Pusan National University, Beomeo-ri, Mulgeum-eup, Yangsan-si, Yangsan, Gyeongsangnam, 626-870, South Korea
3. Department of Electronic and Electrical Engineering, Pohang University of Science and Technology, Pohang, 790-784, South Korea
4. Department of Electrical Engineering, Pusan National University, Busan, 609-735, South Korea
5. Department of Biochemistry, College of Life Science and Biotechnology, Yonsei Univerisity, Seoul, 120-749, South Korea - ISSN：1432-069X
文摘
The barrier system of the skin not only defends against antigens and harmful substances, but also hinders the permeation of medicines and cosmetics into the dermis. Several strategies have been developed to enhance the absorption ability of skin, including the use of chemicals and skin ablation devices. However, the cost and inconvenience of these strategies highlights the need for a novel and safe method for increasing skin absorption. In this study, we examined the effect of low temperature atmospheric pressure plasma (LTAPP) on the efficiency of drug penetration through the skin, as well as its mechanism of action. HaCaT human keratinocytes and hairless mice were exposed to LTAPP treatment, and the cellular and tissue gene expression, and morphological changes were monitored. We found that the LTAPP exposure reduced the expression of E-cadherin in skin cells and led to the loss of cell–cell contacts. The exposure of mouse skin to LTAPP also reduced the expression of E-cadherin and prevented intercellular junction formation within the tissue, leading to enhanced absorption of hydrophilic agents, eosin and epidermal growth factor. The reduction in E-cadherin expression and reduced skin barrier function recovered completely within 3?h of LTAPP exposure. Taken together, these data show that LTAPP can induce a temporal decrease in the skin barrier function by regulating E-cadherin-mediated intercellular interactions, leading to the enhanced transdermal delivery of drugs and cosmetics.