Low-molecular-weight inhibitors of cell differentiation enable efficient growth of mouse iPS cells under feeder-free conditions

详细信息    查看全文

• 作者：Kenichiro Donai (1)
  Akane Inagaki (1)
  Kyoung-Ha So (1)
  Kengo Kuroda (1)
  Hideko Sone (2)
  Masayuki Kobayashi (3)
  Katsuhiko Nishimori (1)
  Tomokazu Fukuda (1)
  
• 关键词：Induced pluripotent stem cells ; Cell culture condition ; Serum ; free ; Feeder ; free ; Low ; molecular ; weight compounds
• 刊名：Cytotechnology
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：67
• 期：2
• 页码：191-197
• 全文大小：2,582 KB
• 参考文献：1. Amit M, Shariki C, Margulets V, Itskovitz-Eldor J (2004) Feeder layer- and serum-free culture of human embryonic stem cells. Biol Reprod 70:837鈥?45. doi:10.1095/biolreprod.103.021147 CrossRef
  2. Bain J, Plater L, Elliott M, Shpiro N, Hastie CJ, McLauchlan H, Klevernic I, Arthur JS, Alessi DR, Cohen P (2007) The selectivity of protein kinase inhibitors: a further update. Biochem J 408:297鈥?15. doi:10.1042/BJ20070797 CrossRef
  3. Besser D (2004) Expression of nodal, lefty-a, and lefty-B in undifferentiated human embryonic stem cells requires activation of Smad2/3. J Biol Chem 279:45076鈥?5084. doi:10.1074/jbc.M404979200 CrossRef
  4. Donai K, Kuroda K, Guo Y, So KH, Sone H, Kobayashi M, Nishimori K, Fukuda T (2013) Establishment of a reporter system to monitor silencing status in induced pluripotent stem cell lines. Anal Biochem 443:104鈥?12. doi:10.1016/j.ab.2013.08.014 CrossRef
  5. Fukusumi H, Shofuda T, Kanematsu D, Yamamoto A, Suemizu H, Nakamura M, Yamasaki M, Ohgushi M, Sasai Y, Kanemura Y (2013) Feeder-free generation and long-term culture of human induced pluripotent stem cells using pericellular matrix of decidua derived mesenchymal cells. PLoS ONE 8:e55226. doi:10.1371/journal.pone.0055226 CrossRef
  6. Furue M, Okamoto T, Hayashi Y, Okochi H, Fujimoto M, Myoishi Y, Abe T, Ohnuma K, Sato GH, Asashima M, Sato JD (2005) Leukemia inhibitory factor as an anti-apoptotic mitogen for pluripotent mouse embryonic stem cells in a serum-free medium without feeder cells. In Vitro Cell Dev Biol Anim 41:19鈥?8. doi:10.1290/0502010.1 CrossRef
  7. Hayashi Y, Chan T, Warashina M, Fukuda M, Ariizumi T, Okabayashi K, Takayama N, Otsu M, Eto K, Furue MK, Michiue T, Ohnuma K, Nakauchi H, Asashima M (2010) Reduction of N-glycolylneuraminic acid in human induced pluripotent stem cells generated or cultured under feeder- and serum-free defined conditions. PLoS ONE 5:e14099. doi:10.1371/journal.pone.0014099 CrossRef
  8. Kitajima H, Niwa H (2010) Clonal expansion of human pluripotent stem cells on gelatin-coated surface. Biochem Biophys Res Commun 396:933鈥?38. doi:10.1016/j.bbrc.2010.05.026 CrossRef
  9. Lin T, Ambasudhan R, Yuan X, Li W, Hilcove S, Abujarour R, Lin X, Hahm HS, Hao E, Hayek A, Ding S (2009) A chemical platform for improved induction of human iPSCs. Nat Methods 6:805鈥?08. doi:10.1038/nmeth.1393 CrossRef
  10. Lu J, Hou R, Booth CJ, Yang SH, Snyder M (2006) Defined culture conditions of human embryonic stem cells. Proc Natl Acad Sci USA 103:5688鈥?693. doi:10.1073/pnas.0601383103 CrossRef
  11. Miyazaki T, Futaki S, Hasegawa K, Kawasaki M, Sanzen N, Hayashi M, Kawase E, Sekiguchi K, Nakatsuji N, Suemori H (2008) Recombinant human laminin isoforms can support the undifferentiated growth of human embryonic stem cells. Biochem Biophys Res Commun 375:27鈥?2. doi:10.1016/j.bbrc.2008.07.111 CrossRef
  12. Navarro-Alvarez N, Soto-Gutierrez A, Yuasa T, Yamatsuji T, Shirakawa Y, Nagasaka T, Sun SD, Javed MS, Tanaka N, Kobayashi N (2008) Long-term culture of Japanese human embryonic stem cells in feeder-free conditions. Cell Transpl 17:27鈥?3 CrossRef
  13. Polychronopoulos P, Magiatis P, Skaltsounis AL, Myrianthopoulos V, Mikros E, Tarricone A, Musacchio A, Roe SM, Pearl L, Leost M, Greengard P, Meijer L (2004) Structural basis for the synthesis of indirubins as potent and selective inhibitors of glycogen synthase kinase-3 and cyclin-dependent kinases. J Med Chem 47:935鈥?46. doi:10.1021/jm031016d CrossRef
  14. Qin XY, Fukuda T, Yang L, Zaha H, Akanuma H, Zeng Q, Yoshinaga J, Sone H (2012) Effects of bisphenol a exposure on the proliferation and senescence of normal human mammary epithelial cells. Cancer Biol Ther 13:296鈥?06. doi:10.4161/cbt.18942 CrossRef
  15. Rodin S, Domogatskaya A, Strom S, Hansson EM, Chien KR, Inzunza J, Hovatta O, Tryggvason K (2010) Long-term self-renewal of human pluripotent stem cells on human recombinant laminin-511. Nat Biotechnol 28:611鈥?15. doi:10.1038/nbt.1620 CrossRef
  16. Rosler ES, Fisk GJ, Ares X, Irving J, Miura T, Rao MS, Carpenter MK (2004) Long-term culture of human embryonic stem cells in feeder-free conditions. Dev Dyn 229:259鈥?74. doi:10.1002/dvdy.10430 CrossRef
  17. Sato N, Meijer L, Skaltsounis L, Greengard P, Brivanlou AH (2004) Maintenance of pluripotency in human and mouse embryonic stem cells through activation of Wnt signaling by a pharmacological GSK-3-specific inhibitor. Nat Med 10:55鈥?3. doi:10.1038/nm979 CrossRef
  18. Sebolt-Leopold JS, Herrera R (2004) Targeting the mitogen-activated protein kinase cascade to treat cancer. Nat Rev Cancer 4:937鈥?47. doi:10.1038/nrc1503 CrossRef
  19. Sommer CA, Stadtfeld M, Murphy GJ, Hochedlinger K, Kotton DN, Mostoslavsky G (2009) Induced pluripotent stem cell generation using a single lentiviral stem cell cassette. Stem Cells 27:543鈥?49. doi:10.1634/stemcells.2008-1075 CrossRef
  20. Sun N, Panetta NJ, Gupta DM, Wilson KD, Lee A, Jia F, Hu S, Cherry AM, Robbins RC, Longaker MT, Wu JC (2009) Feeder-free derivation of induced pluripotent stem cells from adult human adipose stem cells. Proc Natl Acad Sci USA 106:15720鈥?5725. doi:10.1073/pnas.0908450106 CrossRef
  21. Takahashi K, Yamanaka S (2006) Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126:663鈥?76. doi:10.1016/j.cell.2006.07.024 CrossRef
  22. Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131:861鈥?72. doi:10.1016/J.Cell.11.019 CrossRef
  23. Xu C, Inokuma MS, Denham J, Golds K, Kundu P, Gold JD, Carpenter MK (2001) Feeder-free growth of undifferentiated human embryonic stem cells. Nat Biotechnol 19:971鈥?74. doi:10.1038/nbt1001-971 CrossRef
  24. Xu Y, Zhu X, Hahm HS, Wei W, Hao E, Hayek A, Ding S (2010) Revealing a core signaling regulatory mechanism for pluripotent stem cell survival and self-renewal by small molecules. Proc Natl Acad Sci USA 107:8129鈥?134. doi:10.1073/pnas.1002024107 CrossRef
  25. Yamamoto A, Kumakura S, Uchida M, Barrett JC, Tsutsui T (2003) Immortalization of normal human embryonic fibroblasts by introduction of either the human papillomavirus type 16 E6 or E7 gene alone. Int J Cancer 106:301鈥?09. doi:10.1002/ijc.11219 CrossRef
  26. Ying QL, Wray J, Nichols J, Batlle-Morera L, Doble B, Woodgett J, Cohen P, Smith A (2008) The ground state of embryonic stem cell self-renewal. Nature 453:519鈥?23. doi:10.1038/nature06968 CrossRef
  27. Yu J, Vodyanik MA, Smuga-Otto K, Antosiewicz-Bourget J, Frane JL, Tian S, Nie J, Jonsdottir GA, Ruotti V, Stewart R, Slukvin II, Thomson JA (2007) Induced pluripotent stem cell lines derived from human somatic cells. Science 318:1917鈥?920. doi:10.1126/science.1151526 CrossRef
  
• 作者单位：Kenichiro Donai (1)
  Akane Inagaki (1)
  Kyoung-Ha So (1)
  Kengo Kuroda (1)
  Hideko Sone (2)
  Masayuki Kobayashi (3)
  Katsuhiko Nishimori (1)
  Tomokazu Fukuda (1)
  
  1. Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-amamiyamachi, Aoba-ku, Sendai, 981-8555, Japan
  2. Environmental Exposure Research Section, Center for Environmental Risk Research, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki, 305-8506, Japan
  3. Graduate School of Bioresource Sciences, Akita Prefectural University, 241-438 Kaidobata-Nishi, Nakano, Shimoshinjo, Akita, 010-0195, Japan
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Biomedicine
  Biochemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-0778

文摘

Embryonic stem cells and induced pluripotent stem (iPS) cells are usually maintained on feeder cells derived from mouse embryonic fibroblasts (MEFs). In recent years, the cell culture of iPS cells under serum- and feeder-free conditions is gaining attention in overcoming the biosafety issues for clinical applications. In this study, we report on the use of multiple small-molecular inhibitors (i.e., CHIR99021, PD0325901, and Thiazovivin) to efficiently cultivate mouse iPS cells without feeder cells in a chemically-defined and serum-free condition. In this condition, we showed that mouse iPS cells are expressing the Nanog, Oct3/4, and SSEA-1 pluripotent markers, indicating that the culture condition is optimized to maintain the pluripotent status of iPS cells. Without these small-molecular inhibitors, mouse iPS cells required the adaptation period to start the stable cell proliferation. The application of these inhibitors enabled us the shortcut culture method for the cellular adaptation. This study will be useful to efficiently establish mouse iPS cell lines without MEF-derived feeder cells.