Occurrence of black rot of cultivated mushrooms (Flammulina velutipes) caused by Pseudomonas tolaasii in Korea

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• 作者：Hye-Su Han (1)
  Chang-Sung Jhune (1)
  Jong-Chun Cheong (1)
  Jin-A Oh (1)
  Won-Sik Kong (1)
  Jae-Soon Cha (2)
  Chan-Jung Lee (1) lchanj@korea.kr
• 关键词：Black rot – ; Biochemical tests – ; Flammulina velutipes – ; Lipopeptide – ; Pseudomonas tolaasii – ; 16S rRNA gene – ; Fatty acids – ; Pathogenicity – ; rpoB gene
• 刊名：European Journal of Plant Pathology
• 出版年：2012
• 出版时间：July 2012
• 年：2012
• 卷：133
• 期：3
• 页码：527-535
• 全文大小：390.3 KB
• 参考文献：1. Berti, A. D., Greve, N. J. Q., Christensen, H., & Thomas, M. G. (2007). Identification of a biosynthetic gene cluster and the six associated lipopeptides involved in swarming motility of Pseudomonas syringae pv. tomato DC3000. Journal of Bacteriology, 189, 6312–6323.
  2. Borodin, A. M., Danilkovich, A. V., Allikmets, R. L., Rostapshov, V. M., Chernov, I. P., Azhikina, T. L., Monastyrskaya, S., & Sverdlov, D. (1988). Nucleotide sequence of the rpoB gene coding for the beta-subunit of RNA polymerase in Pseudomonas putida. Doklady Biochemistry and Biophysics, 302, 1261–1265.
  3. Brodey, C. L., Rainey, P. B., Tester, M., & Johnstone, K. (1991). Bacterial blotch disease of the cultivated mushroom is caused by an ion-channel forming lipodepsipeptide toxin. Molecular Plant-Microbe Interactions, 4, 407–411.
  4. Brosius, J., Dull, T. J., Sleeter, D., & Noller, H. F. (1981). Gene organisation and primary structure of a ribosomal DNA operon from Escherichia coli. Journal of Molecular Biology, 148, 107–127.
  5. Felsenstein, J. (1985). Confidence limits on phylogenies: an approach using the booststrap. Evolution, 393, 783–791.
  6. Gandy, D. G. (1968). A technique for screening bacteria causing Brown blotch of cultivated mushrooms. In Report of the Glasshouse Crops Research Institute (pp. 150–154).
  7. Godfrey, S. A. C., Harrow, S. A., Marshall, J. W., & Klena, J. D. (2001). Characterization by 16S rRNA sequence analysis of pseudomonads causing blotch disease of cultivated Agaricus bisporus. Applied and Environmental Microbiology, 67, 4316–4323.
  8. Goor, M., Vantomme, R., Swings, J., Gillis, M., De Kersters, K., & Ley, J. (1986). Phenotypic and genotypic diversity of Pseudomonas tolaasii and white line reacting organisms isolated from cultivated mushrooms. Journal of General Microbiology, 132, 2249–2264.
  9. Houdeau, G., & Olivier, J. M. (1989). Pathology of Pleurotus (oyster mushroom). Champignon, 337, 10–14.
  10. Kim, I. G., Someya, T., & Whang, K. S. (2002). The observation and a quantitative evaluation of viable but non-culturable bacteria in potable groundwater using epifluorescence microscopy. The Korean Journal of Microbiology, 38, 180–185.
  11. Kumar, S., Tamura, K., & Nei, M. (2004). MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinformatics, 5, 150–163.
  12. Lee, H. I., Jeong, K. S., & Cha, J. S. (2002). PCR assays for specific and sensitive detection of Pseudomonas tolaasii, the cause of brown blotch disease of mushrooms. Letters in Applied Microbiology, 35, 276–280.
  13. Lincoln, S. P., Fermor, T. R., Stead, D. E., & Sellwood, J. E. (1991). Bacterial soft rot of Agaricus bitorquis. Plant Pathology, 404, 136–144.
  14. Mollet, C., Drancourt, M., & Raoult, D. (1988). Determination of Coxiella burnetii rpoB sequence and its use for phylogenetic analysis. Gene, 207, 97–103.
  15. Mollet, C., Drancourt, M., & Raoult, D. (1997). rpoB sequence analysis as a novel basis of bacterial dentification. Molecular Microbiology, 26, 1005–1011.
  16. Mortishire-Smith, R. J., Nutkins, J. C., & Packman, L. C. (1991). Determination of the structure of an extracellular peptide produced by the mushroom saprotroph Pseudomonas reactans. Tetrahedron, 47, 3645–3654.
  17. Noval, C., Seisdedos, M. T., & de la Cruz, Y. J. I. (1993). Estudio de la posible relaci贸n entre la ca铆da de yemas en peraly la capacidad bacteriana para formar n煤cleos de hielos u originar podredumbre banda. Bolet铆n de Sanidad Vegetal Plagas, 19, 649–661.
  18. O’Riordain, F. (1976). A disease of cultivated mushrooms caused by Pseudomonas agarici Young. Irish Journal of Agricultural Research, 2, 250.
  19. Olivier, J. M., Guillaumes, J., & Martin, D. (1978). Study of a bacterial disease of mushroom caps. In Proc. 4th Int. Conf. Plant Path. Bact. (pp. 903–916). INRA, Angers.
  20. Oyaizu, H., & Komagata, K. (1983). Grouping of Pseudomonas species on the basis of cellular fatty acid composition and the quinone system with special reference to the existence of 3-hydroxy fatty acids. Journal of General and Applied Microbiology, 292, 17–40.
  21. Paine, S. G. (1919). Studies in bacteriosis. II. A brown blotch disease of cultivated mushrooms. Annals of Applied Biology, 5, 206–219.
  22. Palleroni, N. J. (1984). Genus I. Pseudomonas Migula. In N. R. Krieg & J. G. Holt (Eds.), Bergey’s manual of systematic bacteriology, vol 1. Baltimore: Williams and Wilkins.
  23. Qi, Y., Patra, G., Liang, X., Williams, L. E., Rose, S., Redkar, R. J., & DelVecchio, V. G. (2001). Utilization of rpoB gene as a specific chromosomal marker for real-time PCR detection of Bacillus anthracis. Applied and Environmental Microbiology, 67, 3720–3727.
  24. Rainey, F. A., Janssen, P. H., Wild, D. J. C., & Morgan, H. W. (1991a). Isolation and characterization of an obligately anaerobic, polysaccharolytic, extremely thermophilic member of the genus Spirochaeta. Archives of Microbiology, 155, 396–401.
  25. Rainey, P. B., Brodey, C. L., & Johnstone, K. (1991b). Biological properties and spectrum of activity of tolaasin, a lipodepsipeptide toxin produced by the mushroom pathogen Pseudomonas tolaasii. Physiological and Molecular Plant Pathology, 39, 57–70.
  26. Saitou, N., & Nei, M. (1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4, 406–425.
  27. Sajben, E., Manczinger, L., Nagy, A., Kredics, L., & V谩gv枚lgyi, C. (2010). Characterization of pseudomonads isolated from decaying sporocarps of oyster mushroom. Microbiological Research, 166, 255–267.
  28. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids. Technical note 101. Newark: Microbial ID Inc.
  29. Shirata, A., Sugaya, K., Takasugi, M., & Monde, K. (1995). Isolation and biological activity of toxins produced by a Japanese strain of Pseudomonas tolaasii, the pathogen of bacterial rot of cultivated Oyster mushroom. Annals of the Phytopathologicial Society of Japan, 61, 493–502.
  30. Stainer, R. Y., Palleroni, N. J., & Doudoroff, M. (1966). The aerobic pseuomonads: a taxonomic study. Journal of General Microbiology, 43, 159.
  31. Tanprasert, P., & Reed, B. M. (1997). Detection and identification of bacterial contaminants from strawberry runner explants. In Vitro Cellular & Developmental Biology - Plant, 33, 221–226.
  32. Tsuneda, A., Suyama, K., Murakami, S., & Ohira, I. (1995). Occurrence of Pseudo-monas tolaasii on fruiting bodies of Lentinula edodes formed on Quercus logs. Mycoscience, 36, 283–288.
  33. Weisburg, W. G., Barns, S. M., Pelletier, D. A., & Lane, D. J. (1991). 16S ribosomal DNA amplification for phylogenetic study. Journal of Bacteriology, 173, 697–703.
  34. Wong, W. C., & Preece, T. F. (1979). Identification of Pseudomonas tolaasii: the white line in agar and mushroom tissue block rapid pitting test. Journal of Applied Bacteriology, 47, 401–407.
  35. Wong, W. C., Fletcher, J. T., Unsworth, B. A., & Preece, T. F. (1982). A note on ginger blotch, a new disease of the cultivated mushroom Agaricus bisporus. Journal of Applied Bacteriology, 5, 43–48.
  36. Zarkower, P. A., Wuest, P. J., Royse, D. J., & Myers, B. (1983). Phenotypic traits of fluorescent pseudomonads causing bacterial blotch of Agaricus bisporus mushrooms and other mushroom-derived fluorescent pseudomonads. Canadian Journal of Microbiology, 30, 360–367.
  37. Zdor, R. E., & Anderson, A. J. (1992). Influence of root colonizing bacteria on the defense responses of bean. Plant and Soil, 104, 99–107.
• 作者单位：1. Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA, Suwon, 441-707 Korea2. Department of Plant Medicine, College of Agriculture, Life & Environment Science, Chungbuk National University, Cheongju, Chungbuk 631-763, Korea
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Pathology
  Plant Sciences
  Ecology
  
• 出版者：Springer Netherlands
• ISSN：1573-8469

文摘

From black spots on winter mushroom (Flammulina velutipes), fluorescent bacteria were repeatedly isolated during surveys at places of production in the years 2009–2010 in Korea. From these lesions three bacterial strains (designated CHM13, CHM16, CHM17) were isolated which, following inoculation of mushroom stipes and caps, yielded characteristic black spots and sunken lesions, which developed into a severe black rot. Results of Gram stain and biochemical tests preliminarily identified these isolates as Pseudomonas tolaasii. This was confirmed by pathogenicity to winter mushroom, physiological and biochemical properties, analysis of the 16S rRNA and rpoB gene sequences, fatty acids profile, specific and sensitive PCR assays and, lipopeptide detection. This is the first report of the isolation of Pseudomonas tolaasii from cultivated winter mushroom in Korea.