Development and implementation of rapid metabolic engineering tools for chemical and fuel production in ass="a-plus-plus">Geobacillus thermoglucosidasius NCIMB 11955
详细信息 查看全文
- 作者：Lili Sheng ; Katalin Kovács ; Klaus Winzer ; Ying Zhang…
- 关键词：Allelic exchange ; In ; frame deletion ; Counter ; selection marker ; pyrE ; Geobacillus thermoglucosidasius ; Whole ; genome sequencing
- 刊名：Biotechnology for Biofuels
- 出版年：2017
- 出版时间：December 2017
- 年：2017
- 卷：10
- 期：1
- 全文大小：2707KB
- 刊物类别：Chemistry and Materials Science
- 刊物主题：Biotechnology; Plant Breeding/Biotechnology; Environmental Engineering/Biotechnology; Renewable and Green Energy; Renewable and Green Energy; Microbiology;
- 出版者：BioMed Central
- ISSN：1754-6834
- 卷排序：10
文摘
BackgroundThe thermophile Geobacillus thermoglucosidasius has considerable attraction as a chassis for the production of chemicals and fuels. It utilises a wide range of sugars and oligosaccharides typical of those derived from lignocellulose and grows at elevated temperatures. The latter improves the rate of feed conversion, reduces fermentation cooling costs and minimises the risks of contamination. Full exploitation of its potential has been hindered by a dearth of effective gene tools.