Expression parameters of the metabolic pathway genes pyruvate dehydrogenase kinase-1 (PDK-1) and DJ-1/PARK7 in renal cell carcinoma (RCC)

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• 作者：Daniel Baumunk ; Uta Reichelt ; Jannis Hildebrandt ; Hans Krause…
• 关键词：PDK ; 1 ; DJ ; 1/PARK7 ; Renal cell carcinoma ; Glucose metabolism
• 刊名：World Journal of Urology
• 出版年：2013
• 出版时间：October 2013
• 年：2013
• 卷：31
• 期：5
• 页码：1191-1196
• 全文大小：538KB
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• 作者单位：Daniel Baumunk (1) (4)
  Uta Reichelt (3)
  Jannis Hildebrandt (2)
  Hans Krause (2)
  Jan Ebbing (2)
  Hannes Cash (2)
  Kurt Miller (2)
  Martin Schostak (1)
  Steffen Weikert (2)
  
  1. Department of Urology, Universit?tsklinikum Magdeburg A. ?. R., Leipziger Stra?e 44, 39104, Magdeburg, Germany
  4. Klinik für Urologie und Kinderurologie, Universit?tsklinikum Magdeburg A. ?. R., Leipziger Stra?e 44, 39104, Magdeburg, Germany
  3. Department of Pathology, Charité-Universit?tsmedizin Berlin, Charitéplatz 1, 10117, Berlin, Germany
  2. Department of Urology, Charité-Universit?tsmedizin Berlin, Charitéplatz 1, 10117, Berlin
  
• ISSN：1433-8726

文摘

Purpose Metabolic adaptations, such as increases in glucose and energy metabolism, play a pivotal role in the biology of RCC. PDK-1 and DJ-1/PARK7 are thought to control metabolic pathways in cancer. We investigated the expression of PDK-1 and DJ-1/PARK7 in RCC and their prognostic relevance. Methods RCC tumor tissue and corresponding normal parenchyma samples were obtained from 91 patients with clear cell RCC. Expression of PDK-1 and DJ-1/PARK7 was determined on the mRNA and protein levels using quantitative RT-PCR and immunohistochemistry. Expression ratios tumor/normal were analyzed for associations with pathological stage and grade (Kruskal–Wallis ANOVA, chi-square test). Potential associations with progression-free and overall survival were analyzed using Cox regression models. Results PDK-1 mRNA expression was up-regulated as compared to normal tissue (p?<?0.001). Differences were observed by tumor stage (p?<?0.05) with a trend toward lower expression with increasing stage (p?>?0.01). Expression ratio tumor/normal also showed differences by tumor stage with the lowest ratio observed in advanced (pT3) disease. MRNA expression data were confirmed on the protein level with the lowest protein expression in pT3 tumors. PDK-1 expression ratio tumor/normal was inversely associated with outcome after adjustment for stage and grade (HR, 0.54; 95?% CI, 0.31-.94). No associations observed for DJ-1/PARK7 expression. Conclusions PDK is up-regulated in RCC, but down-regulation may be associated with progression toward a metastasizing behavior. Given the role of PDK-1 in the control of glucose metabolism, aerobic glycolysis via up-regulation of PDK-1 may be an early event in RCC development, but less relevant for the progression toward an aggressive phenotype.