Phylogenomic analysis of the Chlamydomonas genome unmasks proteins potentially involved in photosynthetic function and regulation
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- 作者：Arthur R. Grossman ; Steven J. Karpowicz ; Mark Heinnickel ; David Dewez ; Blaise Hamel ; Rachel Dent ; Krishna K. Niyogi ; Xenie Johnson ; Jean Alric and Francis-Andr¨¦ Wollman ; et al.
- 关键词：Chlamydomonas ; GreenCut ; Chloroplast ; Phylogenomics ; Regulation
- 刊名：Photosynthesis Research
- 出版年：2010
- 出版时间：November 2010
- 年：2010
- 卷：106
- 期：1-2
- 页码：3-17
- 全文大小：450.5 KB
文摘
Chlamydomonas reinhardtii, a unicellular green alga, has been exploited as a reference organism for identifying proteins and activities associated with the photosynthetic apparatus and the functioning of chloroplasts. Recently, the full genome sequence of Chlamydomonas was generated and a set of gene models, representing all genes on the genome, was developed. Using these gene models, and gene models developed for the genomes of other organisms, a phylogenomic, comparative analysis was performed to identify proteins encoded on the Chlamydomonas genome which were likely involved in chloroplast functions (or specifically associated with the green algal lineage); this set of proteins has been designated the GreenCut. Further analyses of those GreenCut proteins with uncharacterized functions and the generation of mutant strains aberrant for these proteins are beginning to unmask new layers of functionality/regulation that are integrated into the workings of the photosynthetic apparatus.