Lithotrophic growth of MALL>Sulfurospirillum deleyianum with sulfide as electron donor coupled to respiratory reduction of nitrate to ammonia

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• 作者：Effi Eisenmann ; Joachim Beuerle ; Klaus Sulger ; Peter M. H. Kroneck and Wolfram Schumacher
• 关键词：Sulfide oxidation ; Nitrate ammonification ; Sulfur respiration ; Sulfur reductase ; Sulfurospirillum deleyianum
• 刊名：Archives of Microbiology
• 出版年：1995
• 出版时间：September 1995
• 年：1995
• 卷：164
• 期：3
• 页码：180-185
• 全文大小：59 KB

文摘

Sulfurospirillum deleyianum grew in batch culture under anoxic conditions with sulfide (up to 5 mM) as electron donor, nitrate as electron acceptor, and acetate as carbon source. Nitrate was reduced to ammonia via nitrite, a quantitatively liberated intermediate. Four moles of sulfide were oxidized to elemental sulfur per mole nitrate converted to ammonia. The molar growth yield per mole sulfide consumed, Y_m, was 1.5¡À0.2 g mol−1 for the reduction of nitrate to ammonia. By this type of metabolism,S. deleyianum connected the biogeochemical cycles of sulfur and nitrogen. The sulfur reductase activity inS. deleyianum was inducible, as the activity depended on the presence of sulfide or elemental sulfur during cultivation with nitrate or fumarate as electron acceptor. Hydrogenase activity was always high, indicating that the enzyme is constitutively expressed. The ammonia-forming nitrite reductase was an inducible enzyme, expressed when cells were cultivated with nitrate, nitrite, or elemental sulfur, but repressed after cultivation with fumarate.