Label-free high-throughput and real-time detections of protein interactions by oblique-incidence reflectivity difference method

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• 作者：LiPing He (1)
  Shuang Liu (1)
  Jun Dai (1)
  HuiBin Lü (1)
  KuiJuan Jin (1)
  GuoZhen Yang (1)
  
• 关键词：oblique ; incidence reflectivity difference (OIRD) ; label ; free ; real ; time ; high ; throughput
• 刊名：SCIENCE CHINA Physics, Mechanics & Astronomy
• 出版年：2014
• 出版时间：April 2014
• 年：2014
• 卷：57
• 期：4
• 页码：615-618
• 全文大小：642 KB
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• 作者单位：LiPing He (1)
  Shuang Liu (1)
  Jun Dai (1)
  HuiBin Lü (1)
  KuiJuan Jin (1)
  GuoZhen Yang (1)
  
  1. Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing, 100190, China
  
• ISSN：1869-1927

文摘

Selected Mouse IgG of 1 mg/mL as target was fabricated on microarray for 500 sample dots. Label-free and real-time reaction dynamic processes were detected between the microarrays with Goat Anti-mouse IgG of 0.02 mg/mL using the oblique-incidence reflectivity difference (OIRD) method. We obtained the reaction results and the reaction dynamic curves of 500 protein dots. In addition, we also used label-free detection of protein microarrays of 10080 sample dots, including BSA and different concentrations of Mouse IgG and Rabbit IgG, by OIRD. The obtained reaction results between the protein microarray with 1 mg/mL Goat Anti-mouse IgG and 1 mg/mL Goat Anti-rabbit IgG are reported herein. Experimental results show that OIRD can be not only label-free high-throughput detection method for biological microarrays but also label-free real-time detection in the interaction processes of biomolecules.