Label-free detection repeatability of protein microarrays by oblique-incidence reflectivity difference method

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• 作者：Jun Dai (1)
  Lin Li (2)
  JingYi Wang (1)
  LiPing He (1)
  HuiBin Lu (1)
  KangCheng Ruan (2)
  KuiJuan Jin (1)
  GuoZhen Yang (1)
  
• 关键词：oblique ; incidence reflectivity difference (OIRD) ; protein microarrays ; specificity ; repeatability
• 刊名：SCIENCE CHINA Physics, Mechanics & Astronomy
• 出版年：2012
• 出版时间：December 2012
• 年：2012
• 卷：55
• 期：12
• 页码：2347-2350
• 全文大小：647KB
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• 作者单位：Jun Dai (1)
  Lin Li (2)
  JingYi Wang (1)
  LiPing He (1)
  HuiBin Lu (1)
  KangCheng Ruan (2)
  KuiJuan Jin (1)
  GuoZhen Yang (1)
  
  1. Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing, 100190, China
  2. State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China
  
• ISSN：1869-1927

文摘

We examine the repeatabilities of oblique-incidence reflectivity difference (OIRD) method for label-free detecting biological molecular interaction using protein microarrays. The experimental results show that the repeatabilities are the same in a given microarray or microarray-microarray and are consistent, indicating that OIRD is a promising label-free detection technique for biological microarrays.