Kir1.1 (ROMK) and Kv7.1 (KCNQ1/KvLQT1) are essential for normal gastric acid secretion: importance of functional Kir1.1

详细信息    查看全文

• 作者：Esad Vucic ; Tariq Alfadda…
• 关键词：Stomach ; Parietal cells ; Acid secretion ; Gastric glands ; Ion transport ; H+ ; K+ ; ATPase
• 刊名：Pfl篓鹿gers Archiv - European Journal of Physiology
• 出版年：2015
• 出版时间：July 2015
• 年：2015
• 卷：467
• 期：7
• 页码：1457-1468
• 全文大小：4,574 KB
• 参考文献：1.Asano S, Morii M, Takeguchi N (2004) Molecular and cellular regulation of the gastric proton pump. Biol Pharm Bull 27:1-2PubMed View Article
  2.Boim MA, Ho K, Shuck ME, Bienkowski MJ, Block JH, Slightom JL, Yang Y, Brenner BM, Hebert SC (1995) ROMK inwardly rectifying ATP-sensitive K+ channel. II. Cloning and distribution of alternative forms. Am J Physiol 268:1132-140
  3.Breton S, Alper SL, Gluck SL, Sly WS, Barker JE, Brown D (1995) Depletion of intercalated cells from collecting ducts of carbonic anhydrase II-deficient (CAR2 null) mice. AmJPhysiol 269:761-74
  4.Buharalioglu CK, Akar F (2002) The reactivity of serotonin, acetylcholine and kcl-induced contractions to relaxant agents in the rat gastric fundus. Pharmacol Res 45:325-31PubMed View Article
  5.Cuppoletti J, Sachs G (1984) Regulation of gastric acid secretion via modulation of a chloride conductance. J Biol Chem 259:14952-4959PubMed
  6.Dedek K, Waldegger S (2001) Colocalization of KCNQ1/KCNE channel subunits in the mouse gastrointestinal tract. Pflugers Arch 442:896-02PubMed View Article
  7.Elso CM, Lu X, Culiat CT, Rutledge JC, Cacheiro NL, Generoso WM, Stubbs LJ (2004) Heightened susceptibility to chronic gastritis, hyperplasia and metaplasia in Kcnq1 mutant mice. Hum Mol Genet 13:2813-821PubMed View Article
  8.Forte JG (2004) K+ channels in the secretory membrane of the parietal cell. Focus on "Gastric parietal cell secretory membrane contains PKA- and acid-activated Kir2.1 K+ channels". Am J Physiol Cell Physiol 286:C478–C479PubMed View Article
  9.Forte JG, Hanzel DK, Okamoto C, Chow D, Urushidani T (1990) Membrane and protein recycling associated with gastric HCl secretion. J Intern Med Suppl 732:17-6PubMed View Article
  10.Fujita A, Horio Y, Higashi K, Mouri T, Hata F, Takeguchi N, Kurachi Y (2002) Specific localization of an inwardly rectifying K(+) channel, Kir4.1, at the apical membrane of rat gastric parietal cells; its possible involvement in K(+) recycling for the H(+)-K(+)-pump. J Physiol 540:85-2PubMed Central PubMed View Article
  11.Fujita A, Takeuchi T, Saitoh N, Hanai J, Hata F (2001) Expression of Ca(2+)-activated K(+) channels, SK3, in the interstitial cells of Cajal in the gastrointestinal tract. Am J Physiol 281:C1727–C1733
  12.Geibel JP, Wagner CA, Caroppo R, Qureshi I, Gloeckner J, Manuelidis L, Kirchhoff P, Radebold K (2001) The stomach divalent ion-sensing receptor scar is a modulator of gastric acid secretion. J Biol Chem 276:39549-9552PubMed View Article
  13.Giebisch G, Hebert SC, Wang WH (2003) New aspects of renal potassium transport. Pflugers Arch 446:289-97PubMed
  14.Goswami S, Jain S, Santani D (1997) Anti-ulcer activity of cromakalim (BRL 34915), a potassium-channel opener, against experimentally induced gastric and duodenal ulcers in rats and guinea-pigs. J Pharm Pharmacol 49:195-99PubMed View Article
  15.Grahammer F, Herling AW, Lang HJ, Schmitt-Graff A, Wittekindt OH, Nitschke R, Bleich M, Barhanin J, Warth R (2001) The cardiac K+ channel KCNQ1 is essential for gastric acid secretion. Gastroenterology 120:1363-371PubMed View Article
  16.Hebert SC (2003) Bartter syndrome. Curr Opin Nephrol Hypertens 12:527-32PubMed View Article
  17.Hebert SC, Desir G, Giebisch G, Wang W (2005) Molecular diversity and regulation of renal potassium channels. Physiol Rev 85:319-71PubMed Central PubMed View Article
  18.Hebert SC, Ho K (1994) Structure and functional properties of an inwardly rectifying ATP-regulated K+ channel from rat kidney. Ren Physiol Biochem 17:143-47PubMed
  19.Hebert SC, Wang WH (1997) Structure and function of the low conductance KATP channel. ROMK Wien Klin Wochenschr 109:471-76
  20.Heitzmann D, Grahammer F, von Hahn T, Schmitt-Graff A, Romeo E, Nitschke R, Gerlach U, Lang HJ, Verrey F, Barhanin J, Warth R (2004) Heteromeric KCNE2/KCNQ1 potassium channels in the luminal membrane of gastric parietal cells. J Psychol 561:547-57
  21.Ho K, Nichols CG, Lederer WJ, Lytton J, Vassilev PM, Kanazirska MV, Hebert SC (1993) Cloning and expression of an inwardly rectifying ATP-regulated potassium channel. Nature 362:31-8PubMed View Article
  22.Iwata F, Koo A, Itoh M, Lam K, Leung JW, Leung FW (1997) Functional evidence linking potassium channels and afferent nerve-mediated mucosal protection in rat stomach. Life Sci 61:1713-720PubMed View Article
  23.Jain RN, Brunkan CS, Chew CS, Samuelson LC (2006) Gene expression profiling of gastrin target genes in parietal cells. Physiol Genomics 24:124-32PubMed View Article
  24.Jin W, Klem AM, Lewis JH, Lu Z (1999) Mechanisms of inward-rectifier K+ channel inhibition by tertiapin-Q. Biochemistry 38:14294-4301PubMed View Article
  25.Jin W, Lu Z (1998) A novel high-affinity inhibitor for inward-rectifier K+ channels. Biochemistry 37:13291-3299PubMed View Article
  26.Kanjhan R, Coulson EJ, Adams DJ, Bellingham MC (2005) Tertiapin-Q blocks recombinant and native large conductance K+ channe
• 作者单位：Esad Vucic (1)
  Tariq Alfadda (1)
  Gordon G. MacGregor (2)
  Ke Dong (2)
  Tong Wang (2)
  John P. Geibel (1) (2)
  
  1. Department of Surgery, Yale University School of Medicine, BML, Room 265, New Haven, CT, 06510, USA
  2. Department of Cellular and Molecular Physiology, Yale University School of Medicine, CT, New Haven, 06510, USA
  
• 刊物主题：Human Physiology;
• 出版者：Springer Berlin Heidelberg
• ISSN：1432-2013

文摘

Potassium channels comprise the apical leak pathway supplying extracellular K+ for exchange with protons by the gastric H+, K+-ATPase and provide potential therapeutic targets for inhibiting gastric acid secretion. The Kir1.1 (ROMK) potassium channel mediates the high capacity K+ recycling necessary for NaCl reabsorption in the thick ascending limb of the kidney, and this channel exhibits functional and regulatory characteristic well suited for K+ recycling by gastric parietal cells. We report here that Kir1.1 channels are required for gastric acid secretion and that this channel participates with Kv7.1 (KCNQ1/KvLQT1) in the potassium recycling process. We show that Kir1.1 colocalizes with the β-subunit of H+, K+-ATPase in gastric parietal cells of Kir1.1 wild-type mice. In Kir1.1-deficient mice, gastric mucosal morphology, as well as parietal cell number, proliferation index, and ultrastructure were normal but secretagogue-stimulated gastric acid secretion in whole stomach and perfused gastric glands was absent. Luminal application of potassium-restored acid secretion in perfused gastric glands from Kir1.1-deficient as well as barium-blocked wild-type mice. In wild-type mice, both luminal Tertiapin-Q, an inhibitor of Kir1.1, as well as XE991, an inhibitor of Kv7.1, reduced proton secretion. We propose that Kir1.1 and Kv7.1 channels collaborate in potassium and current recycling across the apical pole of parietal cells.