Identification of a picornavirus from chickens with transmissible viral proventriculitis using metagenomic analysis

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• 作者：Hye-Ryoung Kim ; Se-Joung Yoon ; Hee-Soo Lee ; Yong-Kuk Kwon
• 刊名：Archives of Virology
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：160
• 期：3
• 页码：701-709
• 全文大小：944 KB
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• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Virology
  Medical Microbiology
  Infectious Diseases
  
• 出版者：Springer Wien
• ISSN：1432-8798

文摘

Transmissible viral proventriculitis (TVP), an infectious disease in chickens, is responsible for economic losses in the commercial poultry industry. The major etiologic agent, however, is unknown. Using metagenomics, we compared the diversity of viruses present in proventriculus samples from flocks diagnosed with TVP to those of healthy flocks in South Korea between 2003 and 2012. Each sample had a mean of 21,538,726 sequence reads generated by high-throughput sequencing, with a mean length of 160?nt. Enrichment in viral sequences suggested that at least three viruses were present in each TVP sample. Although we could not determine a pathogen of TVP that matched the known morphology, picornavirus sequences were present in all five disease samples, suggesting an association with TVP. The five samples yielded 1,045-,720?bp contigs with 81-4?%?nt sequence identity to turkey hepatitis virus (accession number: HM751199). Whole-genome analysis indicated that the QIA01 strain of the novel picornavirus was similar to turkey hepatitis virus in the P2 and P3 regions (82.7?%?nt and 95.5?%?aa sequence identity), but different in the structural region and partial 2A peptides (56.2?%?nt and 23.9?%?aa sequence identity). In addition, the QIA01 virus was similar (87.0?%?nt and 95.6?%?aa sequence identity) to chicken megrivirus, recently detected in chickens with malabsorption syndrome in Hungary. Our results are useful for understanding the genetic diversity of avian picornaviruses and for classifying chicken megrivirus as a pathogen affecting the digestive tract of chickens.