Modification of methods for detection of Escherichia coli O157:H7 on produce

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• 作者：Se-Ri Kim (1)
  Yohan Yoon (2)
  Min-Kyoung Seo (1)
  Won-Il Kim (1)
  Won-Bo Shim (3)
  Duck Hwa Chung (3)
  Jong Chul Yun (1)
  Kyoung Yul Ryu (4)
  Byung-Seok Kim (1)
  
• 关键词：selective media ; sample preparation ; Escherichia coli O157 ; H7 ; produce
• 刊名：Food Science and Biotechnology
• 出版年：2014
• 出版时间：August 2014
• 年：2014
• 卷：23
• 期：4
• 页码：1349-1356
• 全文大小：200 KB
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• 作者单位：Se-Ri Kim (1)
  Yohan Yoon (2)
  Min-Kyoung Seo (1)
  Won-Il Kim (1)
  Won-Bo Shim (3)
  Duck Hwa Chung (3)
  Jong Chul Yun (1)
  Kyoung Yul Ryu (4)
  Byung-Seok Kim (1)
  
  1. Microbial Safety Division, Department of Crop Life Safety, National Academy of Agricultural Science, Rural Development Administration, Suwon, Gyeonggi, 441-707, Korea
  2. Department of Food and Nutrition, Sookmyung Women’s University, Seoul, 140-742, Korea
  3. Division of Applied Life Science, Graduate School of Gyeongsang National University, Jinju, Gyeongnam, 660-701, Korea
  4. R&D Evaluation Division, Research Policy Bureau, Rural Development Administration, Suwon, Gyeonggi, 441-707, Korea
  
• ISSN：2092-6456

文摘

To establish the best detection method for Escherichia coli O157:H7 on produce, the sensitivity, specificity, and recovery values of 3 selective media [sorbitol MacConkey agar containing cefixime and tellurite (CTSMAC), sorbitol MacConkey agar containing cefixime, tellurite, and 5-bromo-4-chloro-3-indoxyl-β-glucuronide (CTB-SMAC), and CHROMagar O157 were compared. Sample preparation methods (pummeling, sonication, and hand-shaking) were compared to determine the optimum procedure for qualitative analysis from cherry tomato. The selectivity and recovery rates of stressed cell of the 3 tested media were in the order of CHROMagar O157>CTBSMAC>CT-SMAC. The recovery rates of hand-shaken tomatoes (10/10) were higher than rates of sonicated (8/10) and pummeled tomatoes (6/10) for inoculated cherry tomatoes in modified E. coli (mEC) broth enriched with novobicin. CHROMagar O157 was the optimum agar medium for detection of E. coli O157:H7. Cherry tomatoes should be treated with hand-shaking to enhance recovery for qualitative analysis.