Metabolic engineering to produce γ-linolenic acid in Brassica napus using a Δ6-desaturase from pike eel
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- 作者：Sun Hee Kim ; Kyung Hee Roh ; Kyeong-Ryeol Lee ; Han-Chul Kang…
- 关键词：Brassica napus ; Δ6 ; fatty acid desaturase ; γ ; Linolenic acid ; Muraenesox cinereus
- 刊名：Plant Biotechnology Reports
- 出版年：2016
- 出版时间：November 2016
- 年：2016
- 卷：10
- 期：6
- 页码：475-481
- 全文大小：
- 刊物主题：Plant Sciences; Cell Biology; Plant Biochemistry; Biotechnology; Agriculture;
- 出版者：Springer Japan
- ISSN：1863-5474
- 卷排序：10
文摘
We generated γ-linolenic acid (GLA, C18:3Δ6,9,12)-producing transgenic Brassica napus transformed with McD6DES, the Δ6-desaturase gene identified from pike eel (Muraenesox cinereus) under the control of the seed-specific vicilin promoter. Seed-specific expression of McD6DES in B. napus produced up to 8.4% of GLA by creating a double bond at the sixth position from the carboxyl end of linoleic acid (LA, C18:2Δ9,12) in seeds. These results demonstrate that McD6DES expression enables to reconstitute in polyunsaturated fatty acid biosynthetic pathways, highlighting the potential of GLA biosynthesis as a target for metabolic engineering of oilseed crops.