Halococcus sediminicola sp. nov., an extremely halophilic archaeon isolated from a marine sediment

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• 作者：Kyung June Yim (1) (2)
  In-Tae Cha (3)
  Tae Woong Whon (4)
  Hae-Won Lee (1)
  Hye Seon Song (1) (2)
  Kil-Nam Kim (1)
  Young-Do Nam (5)
  Sung-Jae Lee (4)
  Jin-Woo Bae (4)
  Sung-Keun Rhee (3)
  Jong-Soon Choi (1)
  Myung-Ji Seo (6)
  Seong Woon Roh (1)
  Daekyung Kim (1)
  
• 关键词：Haloarchaea ; Halococcus sediminicola ; Marine sediment ; Polyphasic taxonomy
• 刊名：Antonie van Leeuwenhoek
• 出版年：2014
• 出版时间：January 2014
• 年：2014
• 卷：105
• 期：1
• 页码：73-79
• 全文大小：266 KB
• 作者单位：Kyung June Yim (1) (2)
  In-Tae Cha (3)
  Tae Woong Whon (4)
  Hae-Won Lee (1)
  Hye Seon Song (1) (2)
  Kil-Nam Kim (1)
  Young-Do Nam (5)
  Sung-Jae Lee (4)
  Jin-Woo Bae (4)
  Sung-Keun Rhee (3)
  Jong-Soon Choi (1)
  Myung-Ji Seo (6)
  Seong Woon Roh (1)
  Daekyung Kim (1)
  
  1. Jeju Center and Division of Life Science, Korea Basic Science Institute, Cheju, 690-140, Republic of Korea
  2. Department of Biology, Jeju National University, Cheju, 690-756, Republic of Korea
  3. Department of Microbiology, Chungbuk National University, Ch’ongju, 361-763, Republic of Korea
  4. Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul, 130-701, Republic of Korea
  5. Fermentation and Functionality Research Group, Korea Food Research Institute, Sungnam, 463-746, Republic of Korea
  6. Division of Bioengineering, Incheon National University, Inchon, 406-772, Republic of Korea
  
• ISSN：1572-9699

文摘

A novel, red-pigmented and coccoid haloarchaeon, designated strain CBA1101T, was isolated from a marine sediment. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CBA1101T is most closely related to the genus Halococcus in the family Halobacteriaceae. Strain CBA1101T had a highest 16S rRNA gene sequence similarity of 98.4?% with Halococcus dombrowskii DSM 14522T, followed by 93.7-8.3?% with sequences of other type strains in the genus Halococcus. The RNA polymerase subunit B-gene sequence similarity of strain CBA1101T with that of Halococcus qingdaonensis JCM 13587T is 89.5?% and lower with those of other members of the genus Halococcus. Strain CBA1101T was observed to grow at 25-0?°C, pH 6.0-.0 and in the presence of 15-0?% (w/v) NaCl, with optimal growth at 35-0?°C, pH 7.0 and with 20?% NaCl. The cells of strain CBA1101T are Gram-negative and did not lyse in distilled water. The major polar lipids were identified as phosphatidylglyerol, phosphatidylglycerol phosphate methyl ester, sulfated diglycosyl diether, unidentified phospholipids and unidentified glycolipids. The genomic DNA G+C content was determined 66.0?mol%. The DNA–DNA hybridization experiment showed that there was less than 40?% relatedness between strain CBA1101T and the reference species in the genus Halococcus. Based on this polyphasic taxonomic analysis, strain CBA1101T is considered to represent a new species in the genus Halococcus, for which the name Halococcus sediminicola sp. nov. is proposed. The type strain is CBA1101T (=JCM 18965T?=?CECT 8275T).