Identification of reference genes for tissue-specific gene expression in Panax notoginseng using quantitative real-time PCR

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• 作者：Qiong Wu (1)
  Xinye Ma (2)
  Kefeng Zhang (3)
  Xuehua Feng (1)
  
  1. Guangdong Ecological Engineering Vocational College ; Guangdong Forestry Vocational Technology School ; Guangzhou ; 510520 ; China
  2. Research Center of Chinese Herbal Resource Science and Engineering ; Key Laboratory of Chinese Medicinal Resource from Lingnan ; Ministry of Education ; Guangzhou University of Chinese Medicine ; Guangzhou ; 510006 ; China
  3. College of Pharmacy ; Guilin Medical University ; Guilin ; 541004 ; China
  
• 关键词：Amplification efficiency ; Dammarenediol synthase ; Gene expression stability ; Ginsenosides ; Panax notoginseng ; Primer efficiency
• 刊名：Biotechnology Letters
• 出版年：2015
• 出版时间：January 2015
• 年：2015
• 卷：37
• 期：1
• 页码：197-204
• 全文大小：513 KB
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• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Biotechnology
  Applied Microbiology
  Biochemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-6776

文摘

Validated internal controls are prerequisites to accurately normalize gene expression levels. Here, 14 candidate reference genes in Panax notoginseng were characterized. Primer specificity and amplification efficiency were evaluated for each gene. Candidates were subjected to transcript quantification in the root, fibrous root, rhizome, leaf, receptacle, pedicel, and fruit tissues. Expression stability (M value) and normalization factor variation (Vn/Vn+1) were determined by geNorm. 26S-2 and ACT-2 exhibited the highest expression stability among the tissues. Gene expression of dammarenediol synthase was accurately detected after normalization to 26S-2 and ACT-2 was performed. Results were consistent when each or both of 26S-2 and ACT-2 were applied as internal control. Hence, this study provides useful information to normalize gene expression accurately in the tissue-specific transcripts of P. notoginseng.