Transcription of the var genes from a freshly-obtained field isolate of Plasmodium falciparum shows more variable switching patterns than long laboratory-adapted isolates

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• 作者：Run Ye (1)
  Dongmei Zhang (1)
  Biaobang Chen (1)
  Yongqiang Zhu (2)
  Yilong Zhang (3)
  Shengyue Wang (2)
  Weiqing Pan (1) (3)
  
  1. Department of Tropical Infectious Disease ; Second Military Medical University ; Shanghai ; 200433 ; China
  2. Shanghai-Ministry of Science and Technology Key Laboratory of Health and Disease Genomics ; Chinese National Human Genome Center at Shanghai ; Shanghai ; 201203 ; China
  3. Institute for Infectious Diseases and Vaccine Development ; Tongji University School of Medicine ; Shanghai ; 200092 ; China
  
• 关键词：Plasmodium falciparum ; var gene families ; Switching patterns ; Gene expression ; Transcription profiles ; Wild isolate
• 刊名：Malaria Journal
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：14
• 期：1
• 全文大小：1,922 KB
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• 刊物主题：Parasitology; Infectious Diseases; Tropical Medicine;
• 出版者：BioMed Central
• ISSN：1475-2875

文摘

Background Antigenic variation in Plasmodium falciparum involves switching among multicopy var gene family and is responsible for immune evasion and the maintenance of chronic infections. Current understanding of var gene expression and switching patterns comes from experiments conducted on long laboratory-adapted strains, with little known about their wild counterparts. Methods Genome sequencing was used to obtain 50 var genes from a parasite isolated from the China-Myanmar border. Four clones with different dominant var genes were cultured in vitro in replicates for 50 generations. Transcription of the individual var gene was detected by real-time PCR and then the switching process was analysed. Results The expression of multicopy var genes is mutually exclusive in clones of a wild P. falciparum isolate. The activation of distinct primary dominant var genes leads to different and favoured switching patterns in the four clones. The on/off rates of individual var genes are variable and the choice of subsequent dominant var genes are random, which results in the different switching patterns among replicates of each clonal wild P. falciparum isolate with near identical initial transcription profiles. Conclusions This study suggests that the switching patterns of var genes are abundant, which consist of both conserved and random parts.